Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144226_1zsm AAGGAGAGTTGGTCTCAGAATCCCCAGGCTGG(+AAAAAA)GATCCTGGCTAGCTGGCCCCTTGTCATCAAG NGG 10 8 17 8 24 0 100 5 10
    240606144224_8lom TCCTGCTTGATGACAAGGGGCCAGCTAGCCAG(+AAAAAA)GATCCCAGCCTGGGGATTCTGAGACCAACTC NGG 10 8 17 8 24 0 100 5 10
    240606144223_0zap CACAATGGCAGCTTGTAGCCTTCACCCTTCTG(+AAAAAA)GTACCAGGACTTCATCAACTGAACATTGTTA NGG 10 8 17 8 24 0 100 5 10
    240606144221_6z4b ATGTATAACAATGTTCAGTTGATGAAGTCCTG(+AAAAAA)GTACCAGAAGGGTGAAGGCTACAAGCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240606144219_g6ic TGCTGAACCTCACTCTCCCTTCAACCTCCCCG(+AAAAAA)GATCCAAACCAGCTCCAGGAGGAGGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606144217_jji6 CACCGCCTGTCCCTCCTCCTGGAGCTGGTTTG(+AAAAAA)GATCCGGGGAGGTTGAAGGGAGAGTGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606144216_n2xg ACAGGTCCGTCCCGGAGCACACAGAGGTGTTG(+AAAAAA)GTACCAAGACTGAAACCTTCACAGATTCTAT NGG 10 8 17 8 24 0 100 5 10
    240606144215_uqtl GCCCAATAGAATCTGTGAAGGTTTCAGTCTTG(+AAAAAA)GTACCAACACCTCTGTGTGCTCCGGGACGGA NGG 10 8 17 8 24 0 100 5 10
    240606144213_d0rf GCAGGTCGGGGTTCTCTCAAGTCCCCCAGTCG(+AAAAAA)GATCCGGGACCCCTTTCTGGGCTAGGAAGCG NGG 10 8 17 8 24 0 100 5 10
    240606144211_cave GCGGGCGCTTCCTAGCCCAGAAAGGGGTCCCG(+AAAAAA)GATCCGACTGGGGGACTTGAGAGAACCCCGA NGG 10 8 17 8 24 0 100 5 10