Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144133_wba3 TGCAGGTTTTGAAAGATGTGTCTCATTATCTG(+AAAAAA)GTACCATTCCTGGATCCTCACATTACTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606144131_g4gm CTTTTAAGAAGTAATGTGAGGATCCAGGAATG(+AAAAAA)GTACCAGATAATGAGACACATCTTTCAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606144129_yd3o ACAGGGAAGTTCAAAAGGCACACAGTTGGGGG(+AAAAAA)GATCCTCTATCAGGGGTAGTATAAGTTAAGA NGG 10 8 17 8 24 0 100 5 10
    240606144128_5j4y AACTTTCTTAACTTATACTACCCCTGATAGAG(+AAAAAA)GATCCCCCCAACTGTGTGCCTTTTGAACTTC NGG 10 8 17 8 24 0 100 5 10
    240606144125_vyps CTAGGGTCCCATCGTTTGGTTAAATACCACGG(+AAAAAA)GTACCAGTTAACTCCTGGGCTCTAACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606144124_tu6k GGACCTCTAGGTTAGAGCCCAGGAGTTAACTG(+AAAAAA)GTACCCGTGGTATTTAACCAAACGATGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606144122_4jba CCCTGTCCCAGCCAGCCATAGAGCCTTCCAGG(+AAAAAA)GATCCATCTTCTAAGCGCTCCGCCAGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606144120_rsp6 TCGACAGGGCCTGGCGGAGCGCTTAGAAGATG(+AAAAAA)GATCCCTGGAAGGCTCTATGGCTGGCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606144119_9rzf TTCATTTATCCCATTATAAAACGGAGGTCAGG(+AAAAAA)GATCCCTCCGGGTATCTAACCACGGTCAACT NGG 10 8 17 8 24 0 100 5 10
    240606144117_i4qx GGAAGAGTTGACCGTGGTTAGATACCCGGAGG(+AAAAAA)GATCCCTGACCTCCGTTTTATAATGGGATAA NGG 10 8 17 8 24 0 100 5 10