Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606145307_4sv3 GGAACATAGTATAAATGCTGTGGTATAAAGAG(+AAAAAA)GATCCACATTTTTCATGGAATGAATTCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606145305_i3ns TTCTGAAAAGGAAAAAAAAAAAAGCCTCCTTG(+AAAAAA)GTACCCACACCTGGCTTCTCCACAATCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606145303_wpm4 TGCCAGCTGGATTGTGGAGAAGCCAGGTGTGG(+AAAAAA)GTACCAAGGAGGCTTTTTTTTTTTTCCTTTT NGG 10 8 17 8 24 0 100 5 10
    240606145302_4vp6 TTACCACCGAGGTCACTTCACTGAGGTAACTG(+AAAAAA)GTACCTTAGTCTCTTGTGGCCACTTACAGAT NGG 10 8 17 8 24 0 100 5 10
    240606145300_clvu CCGATATCTGTAAGTGGCCACAAGAGACTAAG(+AAAAAA)GTACCAGTTACCTCAGTGAAGTGACCTCGGT NGG 10 8 17 8 24 0 100 5 10
    240606145259_1yov GCATTCCTCTTGCCCAGCCATGTTCACGGTTG(+AAAAAA)GATCCTTAATGCATTGTGAGTGTTCCATCAA NGG 10 8 17 8 24 0 100 5 10
    240606145258_blg6 AAAGTTTGATGGAACACTCACAATGCATTAAG(+AAAAAA)GATCCAACCGTGAACATGGCTGGGCAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606145256_ud9e TGAGGTTCATTGCTTAAGTCAATGTGGTAGTG(+AAAAAA)GTACCCACTTATAATTCCAGTGTTTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606145255_x5lo CTCAGCCTCCCAAACACTGGAATTATAAGTGG(+AAAAAA)GTACCACTACCACATTGACTTAAGCAATGAA NGG 10 8 17 8 24 0 100 5 10
    240606145253_jm11 TCTCCTCCCCCTCGACCCTCCTAAATAACACG(+AAAAAA)GTACCAGGCTTGCTGGCTGTGGGATTTACAG NGG 10 8 17 8 24 0 100 5 10