Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144027_v432 CGGGTGGCCAAGAACGACGGGGTGCCTCCTAG(+AAAAAA)GATCCCAGGACTTACTGGGGCTTTTCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606144025_1u1d CAGCACAACTGAAAAGCCCCAGTAAGTCCTGG(+AAAAAA)GATCCTAGGAGGCACCCCGTCGTTCTTGGCC NGG 10 8 17 8 24 0 100 5 10
    240606144023_3zjb AAACCAGTCTGTGATGCAGGATGGTTGGCTCG(+AAAAAA)GATCCCGAGGGCGGGCCTATGAGAGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606144021_6jtu AGTGCTCACCTCTCTCATAGGCCCGCCCTCGG(+AAAAAA)GATCCGAGCCAACCATCCTGCATCACAGACT NGG 10 8 17 8 24 0 100 5 10
    240606144019_8tkw TGTGAGGGAAGTCGCTTCCCGCGAGATGCTGG(+AAAAAA)GATCCAGCCTGGTTGTCTTTCCGTAAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606144018_he38 CACAGCCACTTTACGGAAAGACAACCAGGCTG(+AAAAAA)GATCCCAGCATCTCGCGGGAAGCGACTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606144016_kdrk TTTTCCCATCCCAGCTTCCTTCTGGTAAACTG(+AAAAAA)GTACCCTCAGGATAAAGAAGTATATGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240606144015_liub AGGCAGGCACCATATACTTCTTTATCCTGAGG(+AAAAAA)GTACCAGTTTACCAGAAGGAAGCTGGGATGG NGG 10 8 17 8 24 0 100 5 10
    240606144013_h1bk AATAATTTTTTTACAAAAAAAATTCTATCAAG(+AAAAAA)GATCCTTTAAATATCAAGTTTCCCAATGCAC NGG 10 8 17 8 24 0 100 5 10
    240606144012_3m80 TCTAAGTGCATTGGGAAACTTGATATTTAAAG(+AAAAAA)GATCCTTGATAGAATTTTTTTTGTAAAAAAA NGG 10 8 17 8 24 0 100 5 10