Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144100_kw5x TTCAAAGTAACACATTTACCAGGCAAGGCAAG(+AAAAAA)GTACCAGCAGTGATGGAGGAGACTGTATGGA NGG 10 8 17 8 24 0 100 5 10
    240606144059_9u3j AGTCATCCATACAGTCTCCTCCATCACTGCTG(+AAAAAA)GTACCTTGCCTTGCCTGGTAAATGTGTTACT NGG 10 8 17 8 24 0 100 5 10
    240606144057_3ygb TCCCCCTCCCTTCTACCTTTTGCTTGAGACCG(+AAAAAA)GATCCCTCTCTCTATAGCCCAGCCTGGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606144055_t48a AGTTCAAGGCCAGGCTGGGCTATAGAGAGAGG(+AAAAAA)GATCCGGTCTCAAGCAAAAGGTAGAAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606144054_ul0d GTGGAATGTGGCCCTAGGATGTACTGGTTTAG(+AAAAAA)GATCCTAGGAAGGTAATCAATCATCCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606144052_id4s GACACTCTGAGGATGATTGATTACCTTCCTAG(+AAAAAA)GATCCTAAACCAGTACATCCTAGGGCCACAT NGG 10 8 17 8 24 0 100 5 10
    240606144050_igkk TGGACATTCGGGCACAGATAAGATGCTTGCAG(+AAAAAA)GATCCTGTTAGGGTTTTTCTTGCTGGTTCCG NGG 10 8 17 8 24 0 100 5 10
    240606144049_4o8v ATCAACGGAACCAGCAAGAAAAACCCTAACAG(+AAAAAA)GATCCTGCAAGCATCTTATCTGTGCCCGAAT NGG 10 8 17 8 24 0 100 5 10
    240606144048_bimp TCTCTCTCTTCAGACACCAGAAGGTGGCATAG(+AAAAAA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606144046_to2g ACCACATGGTGGCTCACAACCATCTGTAATGG(+AAAAAA)GATCCTATGCCACCTTCTGGTGTCTGAAGAG NGG 10 8 17 8 24 0 100 5 10