Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144009_ztjy AAAAGAGGCAAACCCACAAGTCCCTGCCTTGG(+AAAAAA)GATCCTAGTTCCTAGCAGGGGTCAGCTTATC NGG 10 8 17 8 24 0 100 5 10
    240606144008_imu2 CCTTGGATAAGCTGACCCCTGCTAGGAACTAG(+AAAAAA)GATCCCAAGGCAGGGACTTGTGGGTTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606144005_1d3w CACTGTAGATGCTACAAACATGTCTACCCAGG(+AAAAAA)GTACCACTGAGCTACGTCTTCCTCATGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606144003_1c9m CGTCTGCCCCATGAGGAAGACGTAGCTCAGTG(+AAAAAA)GTACCCTGGGTAGACATGTTTGTAGCATCTA NGG 10 8 17 8 24 0 100 5 10
    240606144002_vurm TTTGCAGCTGTCAGTTGTTCTTTAAACTCTAG(+AAAAAA)GATCCATCTTTGGACCAAAAGTCTGGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606144001_7tsd TAGGCCCAGACCAGACTTTTGGTCCAAAGATG(+AAAAAA)GATCCTAGAGTTTAAAGAACAACTGACAGCT NGG 10 8 17 8 24 0 100 5 10
    240606143959_afg0 GGCTGTACTCTACACACTTCCTAGTCACGCAG(+AAAAAA)GATCCCTGTAGCCAAAACAGTATATGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606143958_jlbw GCAGCTGGAACATATACTGTTTTGGCTACAGG(+AAAAAA)GATCCTGCGTGACTAGGAAGTGTGTAGAGTA NGG 10 8 17 8 24 0 100 5 10
    240606143957_u7fa GACGCCATTGGAGTAGATCACCGCAGGTGACG(+AAAAAA)GTACCAGCCTGAAGCCCCACCCAAAAGGCGC NGG 10 8 17 8 24 0 100 5 10
    240606143955_3zz5 GGTAGGCGCCTTTTGGGTGGGGCTTCAGGCTG(+AAAAAA)GTACCGTCACCTGCGGTGATCTACTCCAATG NGG 10 8 17 8 24 0 100 5 10