Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143938_woxi GACCTCAAGGACAACTCCTGAAAGCCAGCTGG(+AAAAAA)GATCCCAGGAACAGCAGTGAGACCCGGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606143936_u9j3 GTGGGATGACCGGGTCTCACTGCTGTTCCTGG(+AAAAAA)GATCCCAGCTGGCTTTCAGGAGTTGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606143935_p2t5 TCAGAGGTGGGACAGAGAGCTCGGCCACTCAG(+AAAAAA)GATCCATCATGCAAGCAAGCAGGGTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606143934_ck4t CGGCCGGAAGAACCCTGCTTGCTTGCATGATG(+AAAAAA)GATCCTGAGTGGCCGAGCTCTCTGTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240606143932_onp9 CCTGGAAACAACCAGGATCCTATCCATTCAAG(+AAAAAA)GTACCATGGGGCAGGTCCAATCTTCAAACCA NGG 10 8 17 8 24 0 100 5 10
    240606143930_qht4 GCCATTGGTTTGAAGATTGGACCTGCCCCATG(+AAAAAA)GTACCTTGAATGGATAGGATCCTGGTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240606143928_5hp1 TTCAACTTTTAAAACAGAAGAGGCGTTGCTAG(+AAAAAA)GATCCCAGACTCTCTGACATCTAAGGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240606143927_06ww CCTGTGTCGGCCTTAGATGTCAGAGAGTCTGG(+AAAAAA)GATCCTAGCAACGCCTCTTCTGTTTTAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606143925_ynv4 GGTTATAAAGACTGCTATCACCTTTGGCAGTG(+AAAAAA)GTACCAAGGAACAGTATGTATGAATGTGATG NGG 10 8 17 8 24 0 100 5 10
    240606143924_o7z0 AAGATCATCACATTCATACATACTGTTCCTTG(+AAAAAA)GTACCACTGCCAAAGGTGATAGCAGTCTTTA NGG 10 8 17 8 24 0 100 5 10