Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143838_nknc GTTTGAAAGGTGAACTGTAAGACTCTTCACTG(+AAAAAA)GATCCTTAATGTTTCAGTGGTCCAGTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240606143836_kim3 TTTGTTGAAGACTGGACCACTGAAACATTAAG(+AAAAAA)GATCCAGTGAAGAGTCTTACAGTTCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240606143834_1vy0 GGAAGGGCTATCCAGAGACTGCTCCACTTGGG(+AAAAAA)GATCCATCCCATATCCAGTCACCAAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240606143833_65qi AGTGTCTGGATTTGGTGACTGGATATGGGATG(+AAAAAA)GATCCCCAAGTGGAGCAGTCTCTGGATAGCC NGG 10 8 17 8 24 0 100 5 10
    240606143831_o2pn AACAAACAAACAAACAAACATATTATTAGAGG(+AAAAAA)GATCCTACCAAGGCCTTCTGGTCAACTCTAC NGG 10 8 17 8 24 0 100 5 10
    240606143829_wv3w CAAGAGTAGAGTTGACCAGAAGGCCTTGGTAG(+AAAAAA)GATCCCTCTAATAATATGTTTGTTTGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606143827_eefa TACATATGCAGCTAGAGACACAAACTCAGGGG(+AAAAAA)GTACCTGTTAGTTCATATTGTTGTTCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606143826_628q CCTATAGGAGGAACAACAATATGAACTAACAG(+AAAAAA)GTACCCCCTGAGTTTGTGTCTCTAGCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240606143824_e8cs TGTGTCTGTCTTTGGTGGTTGATTATGGTATG(+AAAAAA)GATCCCTGGATGGGACAGTCTCTGCATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606143822_r2dn GGAAAGACCATGCAGAGACTGTCCCATCCAGG(+AAAAAA)GATCCATACCATAATCAACCACCAAAGACAG NGG 10 8 17 8 24 0 100 5 10