Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143820_d956 TTTTGGCCCTGTTGATCTCGACTGAATGCCAG(+AAAAAA)GTACCAGTCTTCTCTACCTTCCCTGCCAAAG NGG 10 8 17 8 24 0 100 5 10
    240606143819_5irl TGAGCCTTTGGCAGGGAAGGTAGAGAAGACTG(+AAAAAA)GTACCTGGCATTCAGTCGAGATCAACAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606143816_e01k AAAAAGCTGCTTCTAGTGTGTGAGGTTTCCGG(+AAAAAA)GTACCTTTTAGTTCCTCATAAAGTTGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606143815_8ara TCAGAGTCTGCAACTTTATGAGGAACTAAAAG(+AAAAAA)GTACCCGGAAACCTCACACACTAGAAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606143814_c6zn AAAAGGTTTGTTTTCTTTCATAATTGCATCTG(+AAAAAA)GTACCCAGCAGGAAGTACCTGGCCCCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606143812_m3my CGAACAAGGAGGGGCCAGGTACTTCCTGCTGG(+AAAAAA)GTACCAGATGCAATTATGAAAGAAAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240606143810_uqeo TGTTATGAAATATAATCTGGTGGCCGGCCGGG(+AAAAAA)GTACCTGGAGGACCCATGCCAAGCTGTGCAA NGG 10 8 17 8 24 0 100 5 10
    240606143808_8kqt CTCAGTTGCACAGCTTGGCATGGGTCCTCCAG(+AAAAAA)GTACCCCGGCCGGCCACCAGATTATATTTCA NGG 10 8 17 8 24 0 100 5 10
    240606143806_74cw CCTTTGACCATCTCTGTTTTGGGTTCAGTTTG(+AAAAAA)GATCCTGAAACTTTCCTTCCCAGAAGGTTTG NGG 10 8 17 8 24 0 100 5 10
    240606143805_g92k GGGCACAAACCTTCTGGGAAGGAAAGTTTCAG(+AAAAAA)GATCCAAACTGAACCCAAAACAGAGATGGTC NGG 10 8 17 8 24 0 100 5 10