Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143710_xc7p CAGTGTATGTAAATCCAAGATGGATCTTGGAG(+AAAAAA)GATCCTGAAGAACTTTGTCATAGATATAGGC NGG 10 8 17 8 24 0 100 5 10
    240606143709_53uo GCTCTGCCTATATCTATGACAAAGTTCTTCAG(+AAAAAA)GATCCTCCAAGATCCATCTTGGATTTACATA NGG 10 8 17 8 24 0 100 5 10
    240606143707_gtnz TGCAAAATTAATAAAATCATTGTTTTTGAGGG(+AAAAAA)GTACCAAATCAGCGTCTGCGTTTTGAACTAC NGG 10 8 17 8 24 0 100 5 10
    240606143706_xtde AATCTGTAGTTCAAAACGCAGACGCTGATTTG(+AAAAAA)GTACCCCTCAAAAACAATGATTTTATTAATT NGG 10 8 17 8 24 0 100 5 10
    240606143704_42fv CCAAGGTCTGGAAACTTCTTAATATGAATGTG(+AAAAAA)GATCCGGAAGCAGCCATTTCCAAAAGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606143702_5pb7 TTGCAGAAGACTTTTGGAAATGGCTGCTTCCG(+AAAAAA)GATCCACATTCATATTAAGAAGTTTCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606143701_k3fx CGTCATTTTCACATTAGGAGTTGGCATTTTGG(+AAAAAA)GTACCTCCCAAAATCATACAGATGAGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606143659_hce8 TCTCAGTTTTCTCATCTGTATGATTTTGGGAG(+AAAAAA)GTACCCAAAATGCCAACTCCTAATGTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606143658_6c0k GTGGTATCAGTGCATTTTTGATGCTGATGCTG(+AAAAAA)GATCCAGGGTTGTATTCATATAAAGATGGAT NGG 10 8 17 8 24 0 100 5 10
    240606143655_l7vi CATCCATCCATCTTTATATGAATACAACCCTG(+AAAAAA)GATCCAGCATCAGCATCAAAAATGCACTGAT NGG 10 8 17 8 24 0 100 5 10