Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143653_mud8 AACTTCGAAAAGTTTTAATGTATGTGTACAGG(+AAAAAA)GTACCAAAGCCATATGACACATGATTATTTT NGG 10 8 17 8 24 0 100 5 10
    240606143652_6253 GTCATAAAATAATCATGTGTCATATGGCTTTG(+AAAAAA)GTACCCTGTACACATACATTAAAACTTTTCG NGG 10 8 17 8 24 0 100 5 10
    240606143651_0ece ATGTTCTTCAGGAAAAAAGAATGTTGAACAAG(+AAAAAA)GTACCAGAAAAGGAACTAGAACACATACTGC NGG 10 8 17 8 24 0 100 5 10
    240606143650_aju9 GGAGTGCAGTATGTGTTCTAGTTCCTTTTCTG(+AAAAAA)GTACCTTGTTCAACATTCTTTTTTCCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606143648_g7s1 TCAAAAAGGAGGAGGAAGGCGTCTTGTATGTG(+AAAAAA)GATCCACAGCAACAGGCGTTCAGTTAACATC NGG 10 8 17 8 24 0 100 5 10
    240606143647_az22 GCATGGATGTTAACTGAACGCCTGTTGCTGTG(+AAAAAA)GATCCACATACAAGACGCCTTCCTCCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606143645_4gxz GGAAACTTCCTACGTGTTCGTAGCTACACTGG(+AAAAAA)GTACCAAGGTGCGCGCCGCCGGCGGTCCAAC NGG 10 8 17 8 24 0 100 5 10
    240606143643_lax5 ACGAAGTTGGACCGCCGGCGGCGCGCACCTTG(+AAAAAA)GTACCCAGTGTAGCTACGAACACGTAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606143641_r44h TAGTAAGCATAAGGGGCTGCCTTCATCTTATG(+AAAAAA)GTACCTTGGACACATCATCCTATGGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240606143639_b64t CTGTCCTGTGACCATAGGATGATGTGTCCAAG(+AAAAAA)GTACCATAAGATGAAGGCAGCCCCTTATGCT NGG 10 8 17 8 24 0 100 5 10