Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143547_599c CCAGCAGCCTGAAAAAATTGAAATGCCCAAGG(+AAAAAA)GATCCACCTTGACTTTTGTTTGTGAGAACAT NGG 10 8 17 8 24 0 100 5 10
    240606143546_df60 TCCTGATGTTCTCACAAACAAAAGTCAAGGTG(+AAAAAA)GATCCCTTGGGCATTTCAATTTTTTCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606143544_g8wi ATGCGAGCCTCTTCCGTGTTTCTGTTATTAAG(+AAAAAA)GTACCTCCACCTGCTGCTCAGAGGCAGCACA NGG 10 8 17 8 24 0 100 5 10
    240606143543_z29f GGAGCTGTGCTGCCTCTGAGCAGCAGGTGGAG(+AAAAAA)GTACCTTAATAACAGAAACACGGAAGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606143541_d9oe AGGTCCCTCCATTCCCCTGTATATGCTAAAAG(+AAAAAA)GTACCATGTTTCCAGGGCTTATCATGTATTT NGG 10 8 17 8 24 0 100 5 10
    240606143540_6jbl AATCTAAATACATGATAAGCCCTGGAAACATG(+AAAAAA)GTACCTTTTAGCATATACAGGGGAATGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606143539_2ver TGACAGAGCCTCTCAGGAGACAGCTGTATCAG(+AAAAAA)GATCCTGTCAGCAAGTACTTCTTGGCATCAG NGG 10 8 17 8 24 0 100 5 10
    240606143538_624d TATTGCTGATGCCAAGAAGTACTTGCTGACAG(+AAAAAA)GATCCTGATACAGCTGTCTCCTGAGAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606143537_rfbh ACAAAGTAAATGGGGGAGATCTCAGCATAGTG(+AAAAAA)GTACCCACCTTTAATCCAAGTACTTGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606143534_fp8o TTCTGCTTCCCAAGTACTTGGATTAAAGGTGG(+AAAAAA)GTACCACTATGCTGAGATCTCCCCCATTTAC NGG 10 8 17 8 24 0 100 5 10