Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143533_hxvx AATATTGTTTGCTGTTTTATGCTAAAGTTGTG(+AAAAAA)GATCCCTAAAGCTACATAAACCTTATTTATA NGG 10 8 17 8 24 0 100 5 10
    240606143531_k1c3 AAAATTATAAATAAGGTTTATGTAGCTTTAGG(+AAAAAA)GATCCACAACTTTAGCATAAAACAGCAAACA NGG 10 8 17 8 24 0 100 5 10
    240606143530_wq3r CAAACATACAGTCTACTCATTCCATAAAAAAG(+AAAAAA)GTACCTTTCCTTCCTTTCCTTTTACTCATCC NGG 10 8 17 8 24 0 100 5 10
    240606143529_5tp4 GAAAGGGATGAGTAAAAGGAAAGGAAGGAAAG(+AAAAAA)GTACCTTTTTTATGGAATGAGTAGACTGTAT NGG 10 8 17 8 24 0 100 5 10
    240606143527_pz7y ACTGAATGGCAGCTGTGGACAGGATCTATGTG(+AAAAAA)GTACCCTAAATGAATAAGTGAGATACTACCC NGG 10 8 17 8 24 0 100 5 10
    240606143526_pcey ACAGGGGGTAGTATCTCACTTATTCATTTAGG(+AAAAAA)GTACCACATAGATCCTGTCCACAGCTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240606143525_857h ATGGATGGAAGAAGACGGTGTCAAGGAATGAG(+AAAAAA)GTACCTGACGCTTCTCGGCATCTCTGCGATC NGG 10 8 17 8 24 0 100 5 10
    240606143524_96bs ATGCAGATCGCAGAGATGCCGAGAAGCGTCAG(+AAAAAA)GTACCTCATTCCTTGACACCGTCTTCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606143522_a60d GTCACCGAGTGTTGGTACTTTATAACTGCATG(+AAAAAA)GTACCCTAGAAATGATCGTTTCATCTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606143521_8c45 CATTGAAAGAAGATGAAACGATCATTTCTAGG(+AAAAAA)GTACCATGCAGTTATAAAGTACCAACACTCG NGG 10 8 17 8 24 0 100 5 10