Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143430_u8o7 TTCTTATTTCAGTATTCCCAGAGCTAAGAATG(+AAAAAA)GTACCTAATATACAGTAAGCCCTCAATAAAT NGG 10 8 17 8 24 0 100 5 10
    240606143429_amv5 TGAGTATTTATTGAGGGCTTACTGTATATTAG(+AAAAAA)GTACCATTCTTAGCTCTGGGAATACTGAAAT NGG 10 8 17 8 24 0 100 5 10
    240606143427_z0se TGAACCTAAGGCACAATAGGAAAGGGAGGTGG(+AAAAAA)GTACCAGTGTGGGTCACAGCCTGAAGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606143426_y8tq GGGGTACAGGCTTCAGGCTGTGACCCACACTG(+AAAAAA)GTACCCACCTCCCTTTCCTATTGTGCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606143425_jswi GGGGATCCAGCATCCTCCTCTGAGCTACTTTG(+AAAAAA)GTACCACATACAGAAGTGTGCATATACATGC NGG 10 8 17 8 24 0 100 5 10
    240606143423_zc6p TTCTTGCATGTATATGCACACTTCTGTATGTG(+AAAAAA)GTACCAAAGTAGCTCAGAGGAGGATGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606143421_kvof CATCACAAACATGGGAAACTCTGGTCCCAGGG(+AAAAAA)GATCCAGCATCCTCCTCTGAGCTACTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240606143419_kuey GTGGTACCAAAGTAGCTCAGAGGAGGATGCTG(+AAAAAA)GATCCCCTGGGACCAGAGTTTCCCATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240606143418_b66h AGAGGCAGACACAGGGAGCAGCTCCAGGAACG(+AAAAAA)GTACCGTGGAGGATTCTGGCCAGGGCATCCC NGG 10 8 17 8 24 0 100 5 10
    240606143415_a2eh ACCCTGGGATGCCCTGGCCAGAATCCTCCACG(+AAAAAA)GTACCGTTCCTGGAGCTGCTCCCTGTGTCTG NGG 10 8 17 8 24 0 100 5 10