Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144619_lzrh GGAGGGACTCTCCTGGATTCAGACCTCCACAG(+AAAAAA)GTACCCTGTGACTACCAAAGTTCAAGGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606144618_4e2i AGCCATTCCCCTTGAACTTTGGTAGTCACAGG(+AAAAAA)GTACCTGTGGAGGTCTGAATCCAGGAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240606144616_700k GGGGGGAGGGCTGCCCAGGATGGGAGGATGTG(+AAAAAA)GTACCACCATTATTGCTTCCTCCTAGGGCAA NGG 10 8 17 8 24 0 100 5 10
    240606144615_sqb2 TCTCATTGCCCTAGGAGGAAGCAATAATGGTG(+AAAAAA)GTACCACATCCTCCCATCCTGGGCAGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606144613_jyb2 GCAGCTCACGTGTACACCCACCCTGGCGGGAG(+AAAAAA)GATCCGACTGAGGCCAGGGGTCTGGTCAGTC NGG 10 8 17 8 24 0 100 5 10
    240606144612_8rl5 CCGAGGACTGACCAGACCCCTGGCCTCAGTCG(+AAAAAA)GATCCTCCCGCCAGGGTGGGTGTACACGTGA NGG 10 8 17 8 24 0 100 5 10
    240606144610_ojf4 TTTCTCTATTGGAGACAATGGAGGCTGACAGG(+AAAAAA)GATCCCAGTGGGAGAGTCAGGCCTCAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240606144608_4vto ACTCTGGGCCTGAGGCCTGACTCTCCCACTGG(+AAAAAA)GATCCCTGTCAGCCTCCATTGTCTCCAATAG NGG 10 8 17 8 24 0 100 5 10
    240606144606_xibl CATGAGCTCATCACCTCTTCATCCTATCCCAG(+AAAAAA)GTACCACGGCCCTTAAGGCAGGGCAGCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606144604_y4sv CAGCTCAAGGCTGCCCTGCCTTAAGGGCCGTG(+AAAAAA)GTACCTGGGATAGGATGAAGAGGTGATGAGC NGG 10 8 17 8 24 0 100 5 10