Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143340_0tqv GTCCTCTACCCCCTGTGATGTTGAGCGTGCTG(+AAAAAA)GATCCTGGGGGCTGTCGGAGGCTGGTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606143337_uwii CTGGCGCTGAACCAGCCTCCGACAGCCCCCAG(+AAAAAA)GATCCAGCACGCTCAACATCACAGGGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240606143335_kd73 AACGGCCTCAACAGGGCCGAGAGGCTGACAGG(+AAAAAA)GTACCTGGAGTCCAGTTACTCACTGGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240606143333_dih4 ACTCAGACAGCCAGTGAGTAACTGGACTCCAG(+AAAAAA)GTACCCTGTCAGCCTCTCGGCCCTGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240606143332_6jcb TCTACCCCATGTGGTCTGCCTCTGGTGTGGTG(+AAAAAA)GTACCTGGTCCTTTTTATACCCCTTCCACCT NGG 10 8 17 8 24 0 100 5 10
    240606143330_9xpa AGCCAAGGTGGAAGGGGTATAAAAAGGACCAG(+AAAAAA)GTACCACCACACCAGAGGCAGACCACATGGG NGG 10 8 17 8 24 0 100 5 10
    240606143328_b7ml CCTTCTGCACTCAGCCTTTCCTTGGGGCTAGG(+AAAAAA)GATCCATCACATGGGGTAAGGCCTAGCCTAA NGG 10 8 17 8 24 0 100 5 10
    240606143327_d3m1 TTGGATTAGGCTAGGCCTTACCCCATGTGATG(+AAAAAA)GATCCCTAGCCCCAAGGAAAGGCTGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240606143326_68ac GGTGGGATGAGTACAGGGCCTTCAGGTCTCAG(+AAAAAA)GATCCCAAAGCCTCTGTTGCCCCATGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240606143324_tb1g TAGTCATCCCCATGGGGCAACAGAGGCTTTGG(+AAAAAA)GATCCTGAGACCTGAAGGCCCTGTACTCATC NGG 10 8 17 8 24 0 100 5 10