Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143235_qpm0 ATATACACTATCTAAGTGGATGGGCTATTCTG(+AAAAAA)GATCCAAGCAAGAACCTGGTGTTCAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606143233_cdel CCCTAATGCTGGTCCTTAAGAGTCCCTGGGTG(+AAAAAA)GATCCCAGCCCTAGTGACCTTGTCTTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240606143231_nt4g AGCCCATGGGAAGACAAGGTCACTAGGGCTGG(+AAAAAA)GATCCACCCAGGGACTCTTAAGGACCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240606143229_5w2q CTATCCTTCATATTAGGATGTTGCAAATGTGG(+AAAAAA)GTACCTTCCCCCTTTTCCTTTCAGAATGAGG NGG 10 8 17 8 24 0 100 5 10
    240606143228_mshb CTCATCCTCATTCTGAAAGGAAAAGGGGGAAG(+AAAAAA)GTACCCACATTTGCAACATCCTAATATGAAG NGG 10 8 17 8 24 0 100 5 10
    240606143226_7iu5 ACAGCTCTGCTAAGCCTGTTGCCCAGTACACG(+AAAAAA)GTACCGGGAGCTCTTGTCCCTTTCCTTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606143224_gzc6 TACCATGGAAAGGAAAGGGACAAGAGCTCCCG(+AAAAAA)GTACCGTGTACTGGGCAACAGGCTTAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606143223_zrl2 GGTCAGCTGTAGCCGACCTGGGAAAGCTAGTG(+AAAAAA)GTACCACACCATGTACTGAAAACCTGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606143222_xj0q TGATAAGGAACAGGTTTTCAGTACATGGTGTG(+AAAAAA)GTACCACTAGCTTTCCCAGGTCGGCTACAGC NGG 10 8 17 8 24 0 100 5 10
    240606143220_b3mz GTTTAAACACTGGGTTAAGATCCTTAATTAAG(+AAAAAA)GTACCCATGTCACGTTGACCCTCCATGTGGA NGG 10 8 17 8 24 0 100 5 10