Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143249_bues CTCTCCAAACATCTTGTGCTCTCCCATGGTTG(+AAAAAA)GTACCTCAGCAAAACTCCATAGGAGTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240606143247_8ffx GAGGATGCTCGGAGTGGCACCAGAGATGGCAG(+AAAAAA)GTACCACCCTGACTCTAGTTGTCTGTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606143245_3tsp GTGAGCAGCAACAGACAACTAGAGTCAGGGTG(+AAAAAA)GTACCTGCCATCTCTGGTGCCACTCCGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606143244_dimr AGTGTGCTGTTGCCTATCTTTTTGACATCTGG(+AAAAAA)GTACCTGTGATGGCCTACAGGAAGCCCACAC NGG 10 8 17 8 24 0 100 5 10
    240606143242_p7j5 ATTTTGTGTGGGCTTCCTGTAGGCCATCACAG(+AAAAAA)GTACCCAGATGTCAAAAAGATAGGCAACAGC NGG 10 8 17 8 24 0 100 5 10
    240606143241_0eee GTGGTAGGTTTTCATGGTTATGACTACATGTG(+AAAAAA)GTACCAGTTCTGTGTTATTTGTTTCCTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606143240_fcjq TGGTAGACCAGGAAACAAATAACACAGAACTG(+AAAAAA)GTACCACATGTAGTCATAACCATGAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240606143239_iccj TACTTTGCTGTATTCAAGTCTGTTTCTCAGTG(+AAAAAA)GATCCAGTGAACTAAATTCTAACATTTGAAC NGG 10 8 17 8 24 0 100 5 10
    240606143237_tn7c AAACTGTTCAAATGTTAGAATTTAGTTCACTG(+AAAAAA)GATCCACTGAGAAACAGACTTGAATACAGCA NGG 10 8 17 8 24 0 100 5 10
    240606143236_ni4y TTTTTTTTTTCTGAACACCAGGTTCTTGCTTG(+AAAAAA)GATCCAGAATAGCCCATCCACTTAGATAGTG NGG 10 8 17 8 24 0 100 5 10