Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143203_ajko GGCTAGAGAGATGGCTCTTCTAGAGGACCAGG(+AAAAAA)GATCCTAGCATCTACATGGCAGCTCACAATT NGG 10 8 17 8 24 0 100 5 10
    240606143202_e5vf CCTGCCCCATCCTAGCACCCCCAGAGATTTAG(+AAAAAA)GTACCAAGAGTAAGGCAGCCCAAGCCAATAT NGG 10 8 17 8 24 0 100 5 10
    240606143201_8s29 GTCACATATTGGCTTGGGCTGCCTTACTCTTG(+AAAAAA)GTACCTAAATCTCTGGGGGTGCTAGGATGGG NGG 10 8 17 8 24 0 100 5 10
    240606143159_cs5x TGGCCTAGACCTTTATATGCTGATCAGGTTGG(+AAAAAA)GTACCAACTGTCAGCCTGTCTGCTGTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606143158_w7f5 GAGATGGGAGACAGCAGACAGGCTGACAGTTG(+AAAAAA)GTACCCAACCTGATCAGCATATAAAGGTCTA NGG 10 8 17 8 24 0 100 5 10
    240606143157_fcnj CAGATGACAGCTTACACCAATCAGGCTGATTG(+AAAAAA)GTACCAAAAAGCACACATTTCTGCTTTTATT NGG 10 8 17 8 24 0 100 5 10
    240606143155_7nyh AATAAAATAAAAGCAGAAATGTGTGCTTTTTG(+AAAAAA)GTACCAATCAGCCTGATTGGTGTAAGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240606143153_o79f GAGCCTCCGCGGAGCCGTCCGTCTGTCCCTGG(+AAAAAA)GATCCTCGAGCGTCTGGCGGTTCCCTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606143151_nk88 TGTGGTGCTGAGGGAACCGCCAGACGCTCGAG(+AAAAAA)GATCCCAGGGACAGACGGACGGCTCCGCGGA NGG 10 8 17 8 24 0 100 5 10
    240606143150_5pvx TCATGACCCATCTTCAGAGACTGACATTTCAG(+AAAAAA)GATCCATATTAAAGCATTCATTTTCTTTGTT NGG 10 8 17 8 24 0 100 5 10