Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143135_1dir CTGTTTCCACCCCCCTCCCACCCGGTTAATAG(+AAAAAA)GATCCATTCATAGCTACTCACTACTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606143133_k5lq GTGCCATCTCTGAATGCAGAACTCATGTCCAG(+AAAAAA)GTACCAGTACTGTAGTGGAGCATAGGATACA NGG 10 8 17 8 24 0 100 5 10
    240606143132_wem6 TGTGCTGTATCCTATGCTCCACTACAGTACTG(+AAAAAA)GTACCTGGACATGAGTTCTGCATTCAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240606143131_nkh3 GTAATCTCTTCCCTCACATGTAACAAACTCTG(+AAAAAA)GATCCAACCCTCAGTACTACGGATCTAAATC NGG 10 8 17 8 24 0 100 5 10
    240606143129_7y5c AGTGAGATTTAGATCCGTAGTACTGAGGGTTG(+AAAAAA)GATCCAGAGTTTGTTACATGTGAGGGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606143128_ja0u TTTCGGGGTCTCTGTTTCTTTGTCTATAAAAG(+AAAAAA)GATCCTTTACTAGGATAGAAAGTTGTGATAT NGG 10 8 17 8 24 0 100 5 10
    240606143126_arjr AAAACATATCACAACTTTCTATCCTAGTAAAG(+AAAAAA)GATCCTTTTATAGACAAAGAAACAGAGACCC NGG 10 8 17 8 24 0 100 5 10
    240606143125_vt4o GGAAGGCCATGCTGTCCACCCTTCCTGGCCAG(+AAAAAA)GATCCAGTTGAGCAGCAGAGGTTGACTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606143123_69pm CCTTCATTTAGTCAACCTCTGCTGCTCAACTG(+AAAAAA)GATCCTGGCCAGGAAGGGTGGACAGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240606143122_7p4l TATGGTTAGGTCTTCAGAACTTAATTAAATTG(+AAAAAA)GATCCTGGAGATGAGGCACATGGGTAGAGAA NGG 10 8 17 8 24 0 100 5 10