Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143031_pwuf GGGAGAGCAGACATCCGCACAATTCATTGTGG(+AAAAAA)GATCCAGGCGGGTGCGGTTCTGTGGATGAGG NGG 10 8 17 8 24 0 100 5 10
    240606143029_hbhu GATGTCAGCGTTTGGATGCTGATTATGGGGTG(+AAAAAA)GATCCCTGGATATGGAAGTCTCTACATGGAC NGG 10 8 17 8 24 0 100 5 10
    240606143027_xcyr GGATGGTCCATGTAGAGACTTCCATATCCAGG(+AAAAAA)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606143025_n3py TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+AAAAAA)GATCCTCCGGTAGTACTATGTCCAATTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606143023_7q0a TCCTCAGGAAATTGGACATAGTACTACCGGAG(+AAAAAA)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606143022_wg6a TTCATTTTCTGCGTGCTCTGACCTTACATTTG(+AAAAAA)GATCCACTCAAAATCTTGCTCCTTGCCTGGT NGG 10 8 17 8 24 0 100 5 10
    240606143020_fzyy ATGTGACCAGGCAAGGAGCAAGATTTTGAGTG(+AAAAAA)GATCCAAATGTAAGGTCAGAGCACGCAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606143019_zrqa GAGTCATTCTGGGAAAACTAACAATTCATAGG(+AAAAAA)GATCCAGGTCCCTGCCCGGTCCATTAAACAA NGG 10 8 17 8 24 0 100 5 10
    240606143017_2xr7 TCCTTTTGTTTAATGGACCGGGCAGGGACCTG(+AAAAAA)GATCCCTATGAATTGTTAGTTTTCCCAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606143015_yhp7 ATATAACACTTGGGGGCAGGGGTGGGTGGGGG(+AAAAAA)GTACCTGTATTGTTCAGATTGCTTCTGAGAA NGG 10 8 17 8 24 0 100 5 10