Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143013_8xt8 CTTGCTTCTCAGAAGCAATCTGAACAATACAG(+AAAAAA)GTACCCCCCACCCACCCCTGCCCCCAAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606143012_ty28 TTTGAAAACAATGAAACAGGATGCTGCTTCTG(+AAAAAA)GTACCCACACCAGCCCCGTTGAACAGATGAT NGG 10 8 17 8 24 0 100 5 10
    240606143010_eocq GGCCTATCATCTGTTCAACGGGGCTGGTGTGG(+AAAAAA)GTACCAGAAGCAGCATCCTGTTTCATTGTTT NGG 10 8 17 8 24 0 100 5 10
    240606143008_6bci TAGTCTCTTGTTCCTTCATAATTGTTCCTTGG(+AAAAAA)GATCCACAACATAGAATTAATCACAGTCGTG NGG 10 8 17 8 24 0 100 5 10
    240606143006_z91h TAAACCACGACTGTGATTAATTCTATGTTGTG(+AAAAAA)GATCCCAAGGAACAATTATGAAGGAACAAGA NGG 10 8 17 8 24 0 100 5 10
    240606143004_34lq AGTGAGGGCTTTGTCCCAAGAAGGCTGTGTGG(+AAAAAA)GTACCAGATTCTGCAAGTGTTGAAGTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240606143003_up85 GAACTATAGAACTTCAACACTTGCAGAATCTG(+AAAAAA)GTACCCACACAGCCTTCTTGGGACAAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240606143001_b6w4 AGGTGGCCATGAGCACCTAATGTGGGTGCTGG(+AAAAAA)GATCCTGAGTCAATGCCTCTGCAAGAGCCGT NGG 10 8 17 8 24 0 100 5 10
    240606143000_7cbn GTGCGACGGCTCTTGCAGAGGCATTGACTCAG(+AAAAAA)GATCCCAGCACCCACATTAGGTGCTCATGGC NGG 10 8 17 8 24 0 100 5 10
    240606142958_6q7r TCCCTTCCTGGATCACAATGTCTGGGGATTAG(+AAAAAA)GATCCATCCATCATCCTAGCCAAGAACTGCA NGG 10 8 17 8 24 0 100 5 10