Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142942_8ijt TTGATGTCACTGCAGAATAGACCAACAATCAG(+AAAAAA)GATCCAGAAAGTTATTCAGTGTTTCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606142940_7v8z AAAAAAAAGCTTGGAAAAGTGAATTTAAAGAG(+AAAAAA)GATCCAAGGTAACATTCTTGAACTAAGATGC NGG 10 8 17 8 24 0 100 5 10
    240606142938_azg4 GGAAGGCATCTTAGTTCAAGAATGTTACCTTG(+AAAAAA)GATCCTCTTTAAATTCACTTTTCCAAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606142937_7dbc TGTCTCAAAGCACAGAAGTCTCAAAACTGTGG(+AAAAAA)GTACCTAGAGTCCCACAATGCCTCACTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606142935_guz3 ATGAAAAGAAGTGAGGCATTGTGGGACTCTAG(+AAAAAA)GTACCCACAGTTTTGAGACTTCTGTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606142934_lkgw CACAGCTCCTTAGTTGCTGGGCAGGGCTCAAG(+AAAAAA)GATCCTTCCCCACTTCATGTGGGATGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606142932_pc5n GTCTGGAACTCATCCCACATGAAGTGGGGAAG(+AAAAAA)GATCCTTGAGCCCTGCCCAGCAACTAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606142931_c11v CTGAACATTTAGAACTCAGAGCCCCATTCATG(+AAAAAA)GTACCTACAGCCCATCTGGAAAATTCTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606142930_6uvu TACTATTGAAGAATTTTCCAGATGGGCTGTAG(+AAAAAA)GTACCATGAATGGGGCTCTGAGTTCTAAATG NGG 10 8 17 8 24 0 100 5 10
    240606142929_npe4 AGGCACCTTCCTGTAGAAGAGCCCATAGTCAG(+AAAAAA)GTACCCAATTACACCTTGGTGTTGTCCAAGA NGG 10 8 17 8 24 0 100 5 10