Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142856_zhji CAGACAGTCCAACAGTAGACAGATAGGTAAGG(+AAAAAA)GATCCAGTAATTGCTCATTCCTTAAGACTGG NGG 10 8 17 8 24 0 100 5 10
    240606142854_4c5o CCTCTCGGGCTAGGAGGGCGCTGGATGGCGGG(+AAAAAA)GTACCCTCCCTCCTGCACCGACTCCACTGAA NGG 10 8 17 8 24 0 100 5 10
    240606142852_181e ACTACTTCAGTGGAGTCGGTGCAGGAGGGAGG(+AAAAAA)GTACCCCGCCATCCAGCGCCCTCCTAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240606142851_dvst ATACACAGTCCGGTGGGGGAGGGGCTGGGCTG(+AAAAAA)GATCCTGGTAGGGGTAAGCGGTAGTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606142849_uskh CTGCAAGCTCCACTACCGCTTACCCCTACCAG(+AAAAAA)GATCCAGCCCAGCCCCTCCCCCACCGGACTG NGG 10 8 17 8 24 0 100 5 10
    240606142847_03tl AAAATAATATTTGGGTGAATGATACTGCCATG(+AAAAAA)GATCCTTTTGTTTAATTTTATATATAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240606142846_j710 TATCCGAGCTTATATATAAAATTAAACAAAAG(+AAAAAA)GATCCATGGCAGTATCATTCACCCAAATATT NGG 10 8 17 8 24 0 100 5 10
    240606142844_cgb7 TCACAAGCAGGTGTTTATGTCTGAAATTGGAG(+AAAAAA)GTACCCATTATCTGAAAATTCCTCTCCCATA NGG 10 8 17 8 24 0 100 5 10
    240606142843_s521 TACCTTATGGGAGAGGAATTTTCAGATAATGG(+AAAAAA)GTACCTCCAATTTCAGACATAAACACCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606142842_0cwd CACTTTAAAACACTGTGGAGCCAGGAATAATG(+AAAAAA)GTACCCACCTGTAATCCCAGCACCTGGGAGA NGG 10 8 17 8 24 0 100 5 10