Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143046_vyjb TACCCATCAACTCCTTGAAAGTCTCAGGAAAG(+AAAAAA)GTACCGTGGCCTACTTGGGACCCTTTAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606143044_ewoh TTCTGGGTCAAGGATGCATCCCTGACAACCAG(+AAAAAA)GATCCCAGAACCTTTGCCTGCTGATCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606143042_tzsp TTAGCTCTCAGATCAGCAGGCAAAGGTTCTGG(+AAAAAA)GATCCTGGTTGTCAGGGATGCATCCTTGACC NGG 10 8 17 8 24 0 100 5 10
    240606143041_f48x TCGGAAGGTTGTTCCATTCCTCCACAGCACTG(+AAAAAA)GTACCTTAAAAGCAAACAAAAACCAACGAGA NGG 10 8 17 8 24 0 100 5 10
    240606143040_oe4b CATAGTCTCGTTGGTTTTTGTTTGCTTTTAAG(+AAAAAA)GTACCAGTGCTGTGGAGGAATGGAACAACCT NGG 10 8 17 8 24 0 100 5 10
    240606143038_646s TAGATGGTCAACTTGATCCTGATTCAACTTTG(+AAAAAA)GTACCTGATATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606143037_swib GACAAATGGACAAATTTCTGGACAGATATCAG(+AAAAAA)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240606143036_kkl8 AGTTTAACTTCTGGAGTCCATATAAAGAGCTG(+AAAAAA)GATCCAAAGTGTGCATTTGCAATCCCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606143035_cvmm GTTCAGTGCTGGGATTGCAAATGCACACTTTG(+AAAAAA)GATCCAGCTCTTTATATGGACTCCAGAAGTT NGG 10 8 17 8 24 0 100 5 10
    240606143033_mygg ATTGCCCTCATCCACAGAACCGCACCCGCCTG(+AAAAAA)GATCCCACAATGAATTGTGCGGATGTCTGCT NGG 10 8 17 8 24 0 100 5 10