Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144358_ya2w ACGGAAGGGTAATTAGGAAGGGTTAAGAGCTG(+AAAAAA)GATCCACGGGCCCAGAGGGAGGTAAGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606144355_2mkr ATGATACACTCTTACCTCCCTCTGGGCCCGTG(+AAAAAA)GATCCAGCTCTTAACCCTTCCTAATTACCCT NGG 10 8 17 8 24 0 100 5 10
    240606144354_1kq4 AGTAGCTGAAAAAAACCAGACAGACAGAGCAG(+AAAAAA)GTACCAGGACTGACTACAGCTCAGGAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240606144352_s452 TAAGATGGACTCCTGAGCTGTAGTCAGTCCTG(+AAAAAA)GTACCTGCTCTGTCTGTCTGGTTTTTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240606144350_rfup GGAAGTAAGTCTTGCTGAAAAGCCACACCAAG(+AAAAAA)GATCCTAGCAGTAGGCGGGTAAGTGACTCCA NGG 10 8 17 8 24 0 100 5 10
    240606144349_gs99 GGGGCTGGAGTCACTTACCCGCCTACTGCTAG(+AAAAAA)GATCCTTGGTGTGGCTTTTCAGCAAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240606144347_xp77 TGCCCATGGTCACTGACCTGAGAGGGGTTAGG(+AAAAAA)GTACCTTTCACAGAAGACAGGGCTCAGTAAA NGG 10 8 17 8 24 0 100 5 10
    240606144346_dsug ACCACTTTACTGAGCCCTGTCTTCTGTGAAAG(+AAAAAA)GTACCCTAACCCCTCTCAGGTCAGTGACCAT NGG 10 8 17 8 24 0 100 5 10
    240606144345_nto4 TTCTCGGTTAAGGTTGGACAGTCATTTCATTG(+AAAAAA)GTACCAGACCACATTTGCAGCAAGATTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606144343_qn4t ACAAGGTGAAATCTTGCTGCAAATGTGGTCTG(+AAAAAA)GTACCAATGAAATGACTGTCCAACCTTAACC NGG 10 8 17 8 24 0 100 5 10