Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143518_vk0t ATAATTCATGGGGGGGGGGGGGCGCGCTAGGG(+AAAAAA)GTACCATGATGGATGTGTGGAGGTCAATGGA NGG 10 8 17 8 24 0 100 5 10
    240606143517_inmc AGTCATCCATTGACCTCCACACATCCATCATG(+AAAAAA)GTACCCCTAGCGCGCCCCCCCCCCCCCATGA NGG 10 8 17 8 24 0 100 5 10
    240606143515_yami GGAAAGGCAGTGGAGGAACTGGGGAGCTCTGG(+AAAAAA)GTACCCAGAATCCTCGAGGTCTCATCTTGAC NGG 10 8 17 8 24 0 100 5 10
    240606143514_z8w3 CAGGTGTCAAGATGAGACCTCGAGGATTCTGG(+AAAAAA)GTACCCAGAGCTCCCCAGTTCCTCCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240606143512_3i57 TGCCAAGAATCACATAGCTGAGGCTATAGATG(+AAAAAA)GTACCGATGACTCTGTGGAACCGTGGTTACA NGG 10 8 17 8 24 0 100 5 10
    240606143511_xvjn TTTATTGTAACCACGGTTCCACAGAGTCATCG(+AAAAAA)GTACCATCTATAGCCTCAGCTATGTGATTCT NGG 10 8 17 8 24 0 100 5 10
    240606143509_2zpy TACGATGGAAATGTATCCTGCCACCTAGCTGG(+AAAAAA)GTACCCATATGGACAGCAACCCAAGGACCCA NGG 10 8 17 8 24 0 100 5 10
    240606143507_59cy TTGTTTGGGTCCTTGGGTTGCTGTCCATATGG(+AAAAAA)GTACCCAGCTAGGTGGCAGGATACATTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606143506_x7ur GCACCTTGACAAAATCAGGGTGTCTAGGGCTG(+AAAAAA)GATCCCCTGAAGCTCTAAGGGAAACTCCATT NGG 10 8 17 8 24 0 100 5 10
    240606143504_kb3z ACCAGAATGGAGTTTCCCTTAGAGCTTCAGGG(+AAAAAA)GATCCAGCCCTAGACACCCTGATTTTGTCAA NGG 10 8 17 8 24 0 100 5 10