Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143501_9wkw CAGGAAAGTAGGATGTGCCCAAGATGCCCATG(+AAAAAA)GATCCTTTGAGAGGCACACAGCCAGGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606143500_k6ho CCTGCCCAGGCCTGGCTGTGTGCCTCTCAAAG(+AAAAAA)GATCCATGGGCATCTTGGGCACATCCTACTT NGG 10 8 17 8 24 0 100 5 10
    240606143459_08hx GATGTGTCTAGCTAGCTAGCCAGAATGCTCAG(+AAAAAA)GATCCTCTATGACCTTATTAGCTTTAACTGT NGG 10 8 17 8 24 0 100 5 10
    240606143457_ke2q AGTTGACAGTTAAAGCTAATAAGGTCATAGAG(+AAAAAA)GATCCTGAGCATTCTGGCTAGCTAGCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240606143456_xnm4 AGTGGCAGGGGTCAGCTTTCTCCATCATGTAG(+AAAAAA)GTACCAGATATCAAACCCAGGTTTTCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240606143455_yw76 TACCCAAACTGAAAACCTGGGTTTGATATCTG(+AAAAAA)GTACCTACATGATGGAGAAAGCTGACCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606143454_h8mb TGTGGGAATCAGTTCTCTTCTTCCAGCATGTG(+AAAAAA)GATCCTGGGACTTGAACTTAGGTCTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606143451_wiif CCATAGCCTGAAGACCTAAGTTCAAGTCCCAG(+AAAAAA)GATCCACATGCTGGAAGAAGAGAACTGATTC NGG 10 8 17 8 24 0 100 5 10
    240606143450_fhx4 AAGTATGCCAGCAGCAGACTCCTGAAGGTCAG(+AAAAAA)GATCCCTCACCTGAACTCCAAAACTATCAAA NGG 10 8 17 8 24 0 100 5 10
    240606143448_cxj1 AGGTCTTTGATAGTTTTGGAGTTCAGGTGAGG(+AAAAAA)GATCCTGACCTTCAGGAGTCTGCTGCTGGCA NGG 10 8 17 8 24 0 100 5 10