Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606143804_azas AAATCACTGACTGTATCCCTGGGGTTGTTCTG(+AAAAAA)GATCCTCTGCATCAGGTCACCTCATGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606143803_vzxd CTCCCTCACCCATGAGGTGACCTGATGCAGAG(+AAAAAA)GATCCAGAACAACCCCAGGGATACAGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606143801_o6s1 GTCTCACTCCCTGCCAGCGGCAGTGCTGACAG(+AAAAAA)GATCCTCCTCAAAGGTGCTTAGCTTTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240606143800_8orc CCGGCTTGCCAAAGCTAAGCACCTTTGAGGAG(+AAAAAA)GATCCTGTCAGCACTGCCGCTGGCAGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240606143758_3fai CTAAGACTGTTGTGCTGTGCTGTCCCAAGGTG(+AAAAAA)GATCCCCTCATTTCTTTTTAAATAAAAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606143756_sc42 TTACTTCTCTTTTATTTAAAAAGAAATGAGGG(+AAAAAA)GATCCACCTTGGGACAGCACAGCACAACAGT NGG 10 8 17 8 24 0 100 5 10
    240606143752_35t3 GGCTATTCTCATGTGCTGTCACAGGCAGCCTG(+AAAAAA)GATCCTTGGTGGTCAGGGGATGACCCAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606143750_sjq8 AGAGGAGCTTGGGTCATCCCCTGACCACCAAG(+AAAAAA)GATCCAGGCTGCCTGTGACAGCACATGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606143749_ch4m GGGTCTTACATGCATGTAGGTTTTCCCTAACG(+AAAAAA)GATCCCCGGTGATTAGACCCTTGGACAACAG NGG 10 8 17 8 24 0 100 5 10
    240606143746_wr8z TTCAGCTGTTGTCCAAGGGTCTAATCACCGGG(+AAAAAA)GATCCGTTAGGGAAAACCTACATGCATGTAA NGG 10 8 17 8 24 0 100 5 10