Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606144153_lsua GCCCTAGAAGAAAGCTACGACAGAAAGAGTGG(+AAAAAA)GTACCTCAGGGGTAGATTAGTGACCTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606144151_la1r TGTCTCTGGGAGGTCACTAATCTACCCCTGAG(+AAAAAA)GTACCCACTCTTTCTGTCGTAGCTTTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606144149_ozpt GCTTTCCACATTATTCCCCTCACACAAGCCTG(+AAAAAA)GATCCCTCACACTAACTATCTAGAGTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606144147_bngv AACACCTGCCACTCTAGATAGTTAGTGTGAGG(+AAAAAA)GATCCAGGCTTGTGTGAGGGGAATAATGTGG NGG 10 8 17 8 24 0 100 5 10
    240606144146_jgrn AAAACACAGAGCAGGGACAGGCATGGTTATAG(+AAAAAA)GATCCTAATGCTGAGTCAGGTGTACCGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606144144_wubw TGTGCCAACCGGTACACCTGACTCAGCATTAG(+AAAAAA)GATCCTATAACCATGCCTGTCCCTGCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606144142_8q0v TCAGTAGATACCACAATGAAGCTGAGCTGGGG(+AAAAAA)GATCCTGGGGCCACATTAGTCAGACAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606144139_o420 AGGGACCAGCTGTCTGACTAATGTGGCCCCAG(+AAAAAA)GATCCCCCAGCTCAGCTTCATTGTGGTATCT NGG 10 8 17 8 24 0 100 5 10
    240606144135_m2na ATGGGCTCATCTGTGCATAGACTCAGCCCTGG(+AAAAAA)GATCCACAGAGAGGCAGATCGCTATGCCTTA NGG 10 8 17 8 24 0 100 5 10
    240606144134_vpuw CTCTGTAAGGCATAGCGATCTGCCTCTCTGTG(+AAAAAA)GATCCCAGGGCTGAGTCTATGCACAGATGAG NGG 10 8 17 8 24 0 100 5 10