Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175236_dvnx CCCACCTGAAGCCTCATGCCACCTGTGGAATG(+AGGACA)GTACCTGCTTCTAGGTACAGAGCCTCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613175235_9bjl CAAAGGGCAGGAGGCTCTGTACCTAGAAGCAG(+AGGACA)GTACCATTCCACAGGTGGCATGAGGCTTCAG NGG 10 8 17 8 24 0 100 5 10
    240613175233_7pi1 CTACAGCCAGAGCCAGTGGGGTCACGACCACG(+AGGACA)GATCCTGTGCAGAAGAGGTGGGGATCATTCG NGG 10 8 17 8 24 0 100 5 10
    240613175231_l3mg GTCCACGAATGATCCCCACCTCTTCTGCACAG(+AGGACA)GATCCGTGGTCGTGACCCCACTGGCTCTGGC NGG 10 8 17 8 24 0 100 5 10
    240613175229_ccuu AAGGTCAGGCCAGGCTACAGCAGGGCATCCTG(+AGGACA)GTACCCAATCAGTGTGGCCGCCTTCCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240613175227_zv2o TGAAGGGCCAGGAAGGCGGCCACACTGATTGG(+AGGACA)GTACCAGGATGCCCTGCTGTAGCCTGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613175226_u4tu CCCCACAGAGAACATGAAGTCACAGTGGAGTG(+AGGACA)GATCCCCTGTTTCTTAGGTATTTGATCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613175224_lohr GAACTCTCTGATCAAATACCTAAGAAACAGGG(+AGGACA)GATCCACTCCACTGTGACTTCATGTTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613175222_94p0 GAAACTGAGGGAATTTGTGGCCTTAAAGCAGG(+AGGACA)GATCCCAGAGTAAAGCTCTCAGGGTTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613175220_qk74 AAGGACCCAGAACCCTGAGAGCTTTACTCTGG(+AGGACA)GATCCCTGCTTTAAGGCCACAAATTCCCTCA NGG 10 8 17 8 24 0 100 5 10