Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613175218_xe5v GAGGCTGGCGGAGGCTGGTGTCTCATCATCGG(+AGGACA)GATCCCCTCCCCAGGACCAGGAGGGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613175216_bzh5 GTCTTCTGCAGCCCTCCTGGTCCTGGGGAGGG(+AGGACA)GATCCCGATGATGAGACACCAGCCTCCGCCA NGG 10 8 17 8 24 0 100 5 10
    240613175214_kvgl CTAGTCTGAAGCCCAGCCCTCCACATCTGGGG(+AGGACA)GTACCAAACCTGCCTTGGGAACTGCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613175213_wzij CTTACTCAGGAGCAGTTCCCAAGGCAGGTTTG(+AGGACA)GTACCCCCAGATGTGGAGGGCTGGGCTTCAG NGG 10 8 17 8 24 0 100 5 10
    240613175211_bybe TTGAGTACACACCACAGGGGGAGTGCTACAGG(+AGGACA)GTACCAGGCTCTGGTGGAAACAACTGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240613175210_h2x6 AACAGGGCACCAGTTGTTTCCACCAGAGCCTG(+AGGACA)GTACCCTGTAGCACTCCCCCTGTGGTGTGTA NGG 10 8 17 8 24 0 100 5 10
    240613175208_v7nq CTGCACGCTGCTCAAAGCACAGCCCCAGAGAG(+AGGACA)GATCCGGAAGGCACACCACACCGGAACTGTT NGG 10 8 17 8 24 0 100 5 10
    240613175207_3ql6 CATAAAACAGTTCCGGTGTGGTGTGCCTTCCG(+AGGACA)GATCCTCTCTGGGGCTGTGCTTTGAGCAGCG NGG 10 8 17 8 24 0 100 5 10
    240613175205_jqv6 TGCCCTTCAGTGGCTAGGGGACAAGAGACAGG(+AGGACA)GATCCCTGAATGCCAACCTGTCCCTAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240613175203_j30m TGGATGTGGCTAGGGACAGGTTGGCATTCAGG(+AGGACA)GATCCCTGTCTCTTGTCCCCTAGCCACTGAA NGG 10 8 17 8 24 0 100 5 10