Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133920_v0uh TACTCAATGCAGCAGTAGAGTGGGCCACGGCG(+AAAAAA)GTACCTGCAGTCCAGCTACCCAGAAGTATGA NGG 10 8 17 8 24 0 100 5 10
    240606133919_uhmh GCCCTGACCCCTTTCCATTAGCCAGAGAAGAG(+AAAAAA)GATCCATATGAAACTCCTGCAAGAGGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606133918_5o1c ATGCTACTTTCCTCTTGCAGGAGTTTCATATG(+AAAAAA)GATCCTCTTCTCTGGCTAATGGAAAGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606133917_98fc AGCCCTTTCCATCAGCAGAGGAAGGGAGAAAG(+AAAAAA)GATCCACTCAGGAAAGTGTAGGAATAGCAAA NGG 10 8 17 8 24 0 100 5 10
    240606133916_jisd AAGAGTTTGCTATTCCTACACTTTCCTGAGTG(+AAAAAA)GATCCTTTCTCCCTTCCTCTGCTGATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606133914_0uqj GCCAGTTCCTAGCTGATCACAGCTTTCCTTAG(+AAAAAA)GATCCAGGAAGGTCTGTGTTTTCTGAGAATA NGG 10 8 17 8 24 0 100 5 10
    240606133912_1h93 AAGACTATTCTCAGAAAACACAGACCTTCCTG(+AAAAAA)GATCCTAAGGAAAGCTGTGATCAGCTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606133910_0ypj TTTAAAAATGAAGCCCAGCTGGGTGTGGTGGG(+AAAAAA)GTACCCTTCTAATCCCAGTACTTGGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606133908_z4hx TTCTCTGCCTCCCAAGTACTGGGATTAGAAGG(+AAAAAA)GTACCCCACCACACCCAGCTGGGCTTCATTT NGG 10 8 17 8 24 0 100 5 10
    240606133906_ghqh GCTGCTTCAACCAGACTGAGCTTGGTGCCTGG(+AAAAAA)GTACCCAGTGATGAGCCAAGTAAGGTAGGCA NGG 10 8 17 8 24 0 100 5 10