Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133937_ryq5 AGGTGAGCAAAAAAGATGGGGACAAGCAGAGG(+AAAAAA)GATCCAGTGTCAACTTCCCTGTGGCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240606133935_4bww AGCCAACAGACTTGAAAAGGCCCCTTGTCTAG(+AAAAAA)GTACCCCACGAAAATAAACTTTGAAAACGTA NGG 10 8 17 8 24 0 100 5 10
    240606133934_yu00 CTGGATACGTTTTCAAAGTTTATTTTCGTGGG(+AAAAAA)GTACCTAGACAAGGGGCCTTTTCAAGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606133932_8a55 GAGAACAGAAGCGCCTGGGGAAGAAGGGTGAG(+AAAAAA)GATCCCCCAAATCAGAGTATGCCTGGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606133930_cqj2 TCAATCACTCCCAGGCATACTCTGATTTGGGG(+AAAAAA)GATCCTCACCCTTCTTCCCCAGGCGCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606133928_f3nl GGGATGGGCTTAGACCATTAAAGGGACCAGGG(+AAAAAA)GATCCTGGCATGTGCAAGTGGTGATGGCCGT NGG 10 8 17 8 24 0 100 5 10
    240606133926_2cfv AACCCACGGCCATCACCACTTGCACATGCCAG(+AAAAAA)GATCCCCTGGTCCCTTTAATGGTCTAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240606133925_yieh GGAAAATGTCTCCGTGGATCACTCAGTGATAG(+AAAAAA)GATCCGCCTCTTGAGTGCTGTCAGTGGCTAG NGG 10 8 17 8 24 0 100 5 10
    240606133923_xemi CTGAACTAGCCACTGACAGCACTCAAGAGGCG(+AAAAAA)GATCCTATCACTGAGTGATCCACGGAGACAT NGG 10 8 17 8 24 0 100 5 10
    240606133922_qvla TACTCTCATACTTCTGGGTAGCTGGACTGCAG(+AAAAAA)GTACCGCCGTGGCCCACTCTACTGCTGCATT NGG 10 8 17 8 24 0 100 5 10