Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134037_bsgn CGGGGGCTAAGAACACTGGCTGTTCTTCTAGG(+AAAAAA)GTACCTGAATTCAATTCCCAGCACCTACATG NGG 10 8 17 8 24 0 100 5 10
    240606134036_b7u5 TCTTCTCAATATCCGTCTACACATGGCATTGG(+AAAAAA)GTACCATCCCTCTCAGTGTCTATTGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606134034_k48m CTCTGAGAGAACAATAGACACTGAGAGGGATG(+AAAAAA)GTACCCAATGCCATGTGTAGACGGATATTGA NGG 10 8 17 8 24 0 100 5 10
    240606134033_8fra CTGGTTTACCTATGGAGGGAAGTGCTAGAATG(+AAAAAA)GATCCTGGACTAAACTCCCCCTGATCACCAC NGG 10 8 17 8 24 0 100 5 10
    240606134031_uu8h ACAGTGTGGTGATCAGGGGGAGTTTAGTCCAG(+AAAAAA)GATCCATTCTAGCACTTCCCTCCATAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240606134030_y5gj CATCAGATGAGAACTCCATTAGCTGGCTAGAG(+AAAAAA)GATCCACATACTTCTCACCAATAGGACTGTA NGG 10 8 17 8 24 0 100 5 10
    240606134029_5a9b AAAGTTACAGTCCTATTGGTGAGAAGTATGTG(+AAAAAA)GATCCTCTAGCCAGCTAATGGAGTTCTCATC NGG 10 8 17 8 24 0 100 5 10
    240606134027_3shq ACTGTAATCTCGTTTCCATGCTGCCGAACCTG(+AAAAAA)GATCCCTATTTTAAACTATATTGATGGAATG NGG 10 8 17 8 24 0 100 5 10
    240606134025_gmok GTGTGCATTCCATCAATATAGTTTAAAATAGG(+AAAAAA)GATCCAGGTTCGGCAGCATGGAAACGAGATT NGG 10 8 17 8 24 0 100 5 10
    240606134024_dizb TTAAGCTGCAAATCTTCTCAGGTGTTCATATG(+AAAAAA)GTACCTATCCATAGATACTCACATAGTAAAA NGG 10 8 17 8 24 0 100 5 10