Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135224_7rc0 CAAAATAACATCTCCAAAACCCATGCCCCCGG(+AAAAAA)GATCCAGTGTGAAGGCATACTACCTATGTTA NGG 10 8 17 8 24 0 100 5 10
    240606135223_c5j0 CACCACCTTGCTACGTGCATGTACCAGGCAAG(+AAAAAA)GTACCAAAGATAACAAATGAAAAACAAACCC NGG 10 8 17 8 24 0 100 5 10
    240606135221_07wx ATCATGGGTTTGTTTTTCATTTGTTATCTTTG(+AAAAAA)GTACCTTGCCTGGTACATGCACGTAGCAAGG NGG 10 8 17 8 24 0 100 5 10
    240606135220_6qka AATCAGCTTGCTTTGTTGGCCTAGATATTTTG(+AAAAAA)GATCCTTTATTGAAATGAGGCAGTGAGATAG NGG 10 8 17 8 24 0 100 5 10
    240606135219_24j0 GGAAACTATCTCACTGCCTCATTTCAATAAAG(+AAAAAA)GATCCAAAATATCTAGGCCAACAAAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240606135218_e3qg AGTGGAAATAATTTGCTGTCGTTTCAGACTTG(+AAAAAA)GATCCTACAAGCCTGTCCGTTCTGTAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606135217_nu02 CAGTACCACTTACAGAACGGACAGGCTTGTAG(+AAAAAA)GATCCAAGTCTGAAACGACAGCAAATTATTT NGG 10 8 17 8 24 0 100 5 10
    240606135215_0w5x TGACAAGGGAGTCGGAAGCTAAGGAGGTGACG(+AAAAAA)GATCCTAATGCTTGTCAAATAAACCGATCGC NGG 10 8 17 8 24 0 100 5 10
    240606135214_gp9z TTTTGGCGATCGGTTTATTTGACAAGCATTAG(+AAAAAA)GATCCGTCACCTCCTTAGCTTCCGACTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606135213_1s4t TATCCGTGCTGGAATGGAATGTTAACTGACAG(+AAAAAA)GATCCTGTGTGTGTGTGTGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10