Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134107_e0fy ACAATAGGGTAATGCTTAATAGAGGGCACATG(+AAAAAA)GATCCATACACTAGACTAACGCGGGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606134105_wacd GGTGGGGGTAACACCTGAGCTAAGAGTGGCAG(+AAAAAA)GTACCTAGAATCCCAGGTGACTGCTTGTGTA NGG 10 8 17 8 24 0 100 5 10
    240606134104_l1rn ACAGATACACAAGCAGTCACCTGGGATTCTAG(+AAAAAA)GTACCTGCCACTCTTAGCTCAGGTGTTACCC NGG 10 8 17 8 24 0 100 5 10
    240606134102_yua8 CAGTGATGCCTAGAAAGATGCAGGGATCTTGG(+AAAAAA)GATCCTGTGGTCTTTACTCCTAATCAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606134100_4kx9 CTTCAAGCTCTGATTAGGAGTAAAGACCACAG(+AAAAAA)GATCCCAAGATCCCTGCATCTTTCTAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606134059_png2 TACAATGGGGGGGGGGGGGGGATGACAGAGTG(+AAAAAA)GATCCTTAAAATGTTAAAGGAGTTGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606134058_fup1 CAGTTAAGCCACAACTCCTTTAACATTTTAAG(+AAAAAA)GATCCACTCTGTCATCCCCCCCCCCCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240606134056_phgc TCCCTGACTTTTTATTAGTATGAAGAAGGGAG(+AAAAAA)GATCCAGAGCATAGTTAGGCAAGCACTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606134055_cahj GCTCAGTGAAGTGCTTGCCTAACTATGCTCTG(+AAAAAA)GATCCTCCCTTCTTCATACTAATAAAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606134054_s90k TTTTTTTTTTTTTTTAATTCTTAGTTGTTTGG(+AAAAAA)GATCCTTAAGCATGCAAAAGCTTTGAACAGG NGG 10 8 17 8 24 0 100 5 10