Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134151_0qyf ACATATAAGGTCAGTGTCTCTGATCCGCGCCG(+AAAAAA)GATCCGCACTGCCAGCTGTGATTGGGTTCGA NGG 10 8 17 8 24 0 100 5 10
    240606134149_9elq AGTGTGGAGTATGGTTGGCAAAGACAGGATGG(+AAAAAA)GATCCAGATTGAATACAGCATGGAATGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606134148_na2f TAAGGAAAACATTCCATGCTGTATTCAATCTG(+AAAAAA)GATCCCATCCTGTCTTTGCCAACCATACTCC NGG 10 8 17 8 24 0 100 5 10
    240606134147_hkl1 ATAGTGATTAAATGAGCATTCCTCACTTTTCG(+AAAAAA)GTACCTAGCATAAAGTCTGCATGTCAGTTTA NGG 10 8 17 8 24 0 100 5 10
    240606134145_vrzq AGCAATAAACTGACATGCAGACTTTATGCTAG(+AAAAAA)GTACCGAAAAGTGAGGAATGCTCATTTAATC NGG 10 8 17 8 24 0 100 5 10
    240606134144_veks TTCCAAGTGAAGTCTGCTAAGCAGACCTCAGG(+AAAAAA)GTACCTGGCAGAGCCTGTGTGAACTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606134142_zstr CATTCACACCCAGTTCACACAGGCTCTGCCAG(+AAAAAA)GTACCCTGAGGTCTGCTTAGCAGACTTCACT NGG 10 8 17 8 24 0 100 5 10
    240606134141_eja3 GTGCAAATTTCCAGAAAATAATGGAGATACAG(+AAAAAA)GTACCATAGGACAGTGAAGCAAGAATGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606134139_255r CTGGAGAGACATTCTTGCTTCACTGTCCTATG(+AAAAAA)GTACCTGTATCTCCATTATTTTCTGGAAATT NGG 10 8 17 8 24 0 100 5 10
    240606134138_ylhh GTTTTAGTTTACAAAAGAAAAATCAATGATTG(+AAAAAA)GTACCTTTTTTTAACACTCTCAGATTCCTGA NGG 10 8 17 8 24 0 100 5 10