Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134253_6ymz TCAGAGGTCCAGCCACGGGGACTCCTGCCTGG(+AAAAAA)GTACCATTGTTGTGAAATTCATTAATCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606134251_o9h7 CTCTAGATACAGCTGTGCCTGGAGGGCCACTG(+AAAAAA)GATCCCCACCCGTTAGATCTCGAGGGCATCA NGG 10 8 17 8 24 0 100 5 10
    240606134249_harp AGACCTGATGCCCTCGAGATCTAACGGGTGGG(+AAAAAA)GATCCAGTGGCCCTCCAGGCACAGCTGTATC NGG 10 8 17 8 24 0 100 5 10
    240606134248_cuwj TCTGGACCCAAGGCTAGAATGTCTCCAGCTGG(+AAAAAA)GATCCCTGAGTTGGGCAGGCCCAACCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606134246_kzuy GCCACTGGAGGGTTGGGCCTGCCCAACTCAGG(+AAAAAA)GATCCCAGCTGGAGACATTCTAGCCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606134245_5hs9 AATCACATTCTCTGTTCAGCCGTTAATGCTGG(+AAAAAA)GATCCATATTTAGTTTTATAAGCTTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606134244_1lvf AAAACAGGGAAAAGCTTATAAAACTAAATATG(+AAAAAA)GATCCCAGCATTAACGGCTGAACAGAGAATG NGG 10 8 17 8 24 0 100 5 10
    240606134242_rom7 TAGTCACTTCCCTCACCAGTGCAGGTGGGTGG(+AAAAAA)GTACCAACCATGCCTCCTCCCCCCCTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606134240_8zvf CCCCCAAAAAAGGGGGGGAGGAGGCATGGTTG(+AAAAAA)GTACCCACCCACCTGCACTGGTGAGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606134239_dhcf GGGCAAGCCGGTTCTCTCCTTCCACCAGATGG(+AAAAAA)GATCCCAGGGGATTGAATTCAGGTTATCTGG NGG 10 8 17 8 24 0 100 5 10