Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134353_c3uv CTTGTATACTGCAAGACAGGTCACACACCAAG(+AAAAAA)GATCCTTCAAACACAAACGCTCCTCCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606134352_m3w2 ATCAAGAAGGGGAGGAGCGTTTGTGTTTGAAG(+AAAAAA)GATCCTTGGTGTGTGACCTGTCTTGCAGTAT NGG 10 8 17 8 24 0 100 5 10
    240606134351_ohta TCAAGAGGCAAAGTCTCCACTTTCCTACACAG(+AAAAAA)GATCCATCTTTTGTCTCAGCCAACTCTCAAT NGG 10 8 17 8 24 0 100 5 10
    240606134350_lf4s CCTCCATTGAGAGTTGGCTGAGACAAAAGATG(+AAAAAA)GATCCTGTGTAGGAAAGTGGAGACTTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606134348_iajj GGGAAGAACTGTTAAGTATCGCTAGGGTCAGG(+AAAAAA)GATCCTGACACCTTGGTCAGCTACCAAACAC NGG 10 8 17 8 24 0 100 5 10
    240606134347_nfq3 CAAGAGTGTTTGGTAGCTGACCAAGGTGTCAG(+AAAAAA)GATCCCTGACCCTAGCGATACTTAACAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606134346_8mzr GCTCCAGTGCCTACAGCTGCAGGGTGGCATTG(+AAAAAA)GATCCTGTGACTCCCGGTCCTTCCTAAGTGA NGG 10 8 17 8 24 0 100 5 10
    240606134344_19vc TCCTTTCACTTAGGAAGGACCGGGAGTCACAG(+AAAAAA)GATCCAATGCCACCCTGCAGCTGTAGGCACT NGG 10 8 17 8 24 0 100 5 10
    240606134343_w0sy TTTAATTAAAGAGAATTCTTCCACTTCGTCGG(+AAAAAA)GATCCGTCTTCTGCAATCAAAGTGGACTGCT NGG 10 8 17 8 24 0 100 5 10
    240606134342_z483 CCTCAAGCAGTCCACTTTGATTGCAGAAGACG(+AAAAAA)GATCCCGACGAAGTGGAAGAATTCTCTTTAA NGG 10 8 17 8 24 0 100 5 10