Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134325_7z0z AAAGGCAGCTGTGAGCTGCTCTGGATGTGGGG(+AAAAAA)GTACCCTGGAGCCCTGGCTGTGGTGACATCA NGG 10 8 17 8 24 0 100 5 10
    240606134323_xbj8 GTTTTATTAGAAAACAAGCTAGGTGGGGAAGG(+AAAAAA)GATCCTTCCAAACCACCATGGAGGTTTGATA NGG 10 8 17 8 24 0 100 5 10
    240606134322_7syq TAAATTATCAAACCTCCATGGTGGTTTGGAAG(+AAAAAA)GATCCCTTCCCCACCTAGCTTGTTTTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240606134321_kr21 TTGTTCTAGAAGGCGCTCCTTTCAGGGTTGTG(+AAAAAA)GTACCTGTAGGTTAGCAGACCCTCCTCCTTT NGG 10 8 17 8 24 0 100 5 10
    240606134319_bjtf GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+AAAAAA)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240606134318_fmx5 CCTTCTACCAAGTGGGTAGTCCGTCCTGATAG(+AAAAAA)GTACCTTTACCTGCTGAGCCATCTGAGTAGC NGG 10 8 17 8 24 0 100 5 10
    240606134317_l8jf GTGAGGCTACTCAGATGGCTCAGCAGGTAAAG(+AAAAAA)GTACCTATCAGGACGGACTACCCACTTGGTA NGG 10 8 17 8 24 0 100 5 10
    240606134316_yecp CAGTGGGTTGCTGCTCTCCAGGGATGTGGCTG(+AAAAAA)GTACCCCAGGTTCTTCCAGGTCATTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606134313_cjkc AGAGGCAGGAGAATGACCTGGAAGAACCTGGG(+AAAAAA)GTACCAGCCACATCCCTGGAGAGCAGCAACC NGG 10 8 17 8 24 0 100 5 10
    240606134312_f3s9 GTGGACTGCATCCGACTGAAATAAGCAAAATG(+AAAAAA)GTACCACAGCGCCCGGGTGCACACAGCAGCC NGG 10 8 17 8 24 0 100 5 10