Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135629_0fv7 TAGGCAGATCACCAGAGCATCTGAGAACAGAG(+AAAAAA)GATCCTGGAAGTTTCGGGGTCTAGAGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240606135627_k67p TCACTCAGGATATTGTTACAGGACATGCAGGG(+AAAAAA)GATCCAATCCCTTCCACACCTTCCGACCCTT NGG 10 8 17 8 24 0 100 5 10
    240606135626_lea9 AGGAGAAGGGTCGGAAGGTGTGGAAGGGATTG(+AAAAAA)GATCCCCTGCATGTCCTGTAACAATATCCTG NGG 10 8 17 8 24 0 100 5 10
    240606135625_mc8l AACAACTAAGATGCTTCATTGTTTCATTTGAG(+AAAAAA)GATCCCAGATCACACAGCACACAAGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606135623_u3fj AGGTACCTGTCCTTGTGTGCTGTGTGATCTGG(+AAAAAA)GATCCTCAAATGAAACAATGAAGCATCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606135621_l28m ACTCTTAAGCTTGACAGGCGGTTGAAGCCCTG(+AAAAAA)GTACCCACATCTGGGGCTAGGTGAACAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606135619_n590 GTACCCTGCTGTTCACCTAGCCCCAGATGTGG(+AAAAAA)GTACCAGGGCTTCAACCGCCTGTCAAGCTTA NGG 10 8 17 8 24 0 100 5 10
    240606135618_tmnx TTGAGATACCGAATGCCTCCCTTTGTGTTTAG(+AAAAAA)GATCCTCAGGACGCTTGGCTCCAGTGCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606135616_x351 AGGAAGCAAGCACTGGAGCCAAGCGTCCTGAG(+AAAAAA)GATCCTAAACACAAAGGGAGGCATTCGGTAT NGG 10 8 17 8 24 0 100 5 10
    240606135614_ogrs AGGCTCTGAATCACAAGTGGACCCGCCCTCAG(+AAAAAA)GATCCAGAGGGAACTGCTCGGATGCCTTACG NGG 10 8 17 8 24 0 100 5 10