Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134515_tg7y TAATATGCAGAATCCTCTACCTGAGAAAAATG(+AAAAAA)GTACCCTGAGAGCAGAAGTGCCTTGCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240606134513_4g7q TGGAAGACAGGCAAGGCACTTCTGCTCTCAGG(+AAAAAA)GTACCATTTTTCTCAGGTAGAGGATTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240606134511_klme ATAACCCCAGAGTTGGGAGATAAAGACAAGAG(+AAAAAA)GATCCATGGGAATTGTAGTTCAACCAGTTTA NGG 10 8 17 8 24 0 100 5 10
    240606134509_he3r TTGGCTAAACTGGTTGAACTACAATTCCCATG(+AAAAAA)GATCCTCTTGTCTTTATCTCCCAACTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606134508_15fg AGTGTCTGGGTTTGGTGGTTGTATATGGGATG(+AAAAAA)GATCCCTGGGTGGGGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606134505_2ei4 GGAAAGACCATCCAGAGACTGCCCCACCCAGG(+AAAAAA)GATCCATCCCATATACAACCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606134503_c5jo TGCTAAGTTCAGGGATGGGAGAATTGCACAAG(+AAAAAA)GTACCTCTTGGGCTGGGAAGTTTAGCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240606134502_e7bo CATCCCAAAAGCTAAACTTCCCAGCCCAAGAG(+AAAAAA)GTACCTTGTGCAATTCTCCCATCCCTGAACT NGG 10 8 17 8 24 0 100 5 10
    240606134501_9byj TACAGACCCAGTCTCCAGCTGTTTTGACACTG(+AAAAAA)GATCCTTGTAAAACTGTACAAGTCATTTGTA NGG 10 8 17 8 24 0 100 5 10
    240606134500_gs1f TGAATTACAAATGACTTGTACAGTTTTACAAG(+AAAAAA)GATCCAGTGTCAAAACAGCTGGAGACTGGGT NGG 10 8 17 8 24 0 100 5 10