Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134559_ddkj AGACTGCTGGGAAACCCAGGCCAAAGGGCAGG(+AAAAAA)GATCCAGACTGGCCATCTCATTGTACACTGT NGG 10 8 17 8 24 0 100 5 10
    240606134558_4cg5 ATGACACAGTGTACAATGAGATGGCCAGTCTG(+AAAAAA)GATCCCTGCCCTTTGGCCTGGGTTTCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606134556_r5q2 GCATGGATTTGATCATGGGGGCCAGGGGCCAG(+AAAAAA)GTACCCCTGACCCTCTCAGTGTGGAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240606134555_8qap AGGTGCATCTCTCCACACTGAGAGGGTCAGGG(+AAAAAA)GTACCTGGCCCCTGGCCCCCATGATCAAATC NGG 10 8 17 8 24 0 100 5 10
    240606134553_47vh ACTTGCTTTTTTTTTTTTTTAATTTGATGCTG(+AAAAAA)GATCCAGAATAGGCCAGTGAAGATCAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606134552_080e ACCACTGCTCTGATCTTCACTGGCCTATTCTG(+AAAAAA)GATCCAGCATCAAATTAAAAAAAAAAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606134550_25k4 TAACTTGAACTTGCAGGTCACCTCTGAACAGG(+AAAAAA)GTACCCTTGTCCCCACCCCAGGCCCTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240606134549_mhjd ACAGGGTAACAGGGCCTGGGGTGGGGACAAGG(+AAAAAA)GTACCCTGTTCAGAGGTGACCTGCAAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606134546_i5g1 TCTCCTGTGTAGACTAGCTGGAGGTCCCTGGG(+AAAAAA)GATCCCCAGGGAAAGGACGCTCTGCCGCGCA NGG 10 8 17 8 24 0 100 5 10
    240606134544_4gea GAGATTGCGCGGCAGAGCGTCCTTTCCCTGGG(+AAAAAA)GATCCCCAGGGACCTCCAGCTAGTCTACACA NGG 10 8 17 8 24 0 100 5 10