Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134631_gbem AACTTCAAAGCTTTATTCTGTCTGCCTGAGAG(+AAAAAA)GATCCATGGAAGATGATTCTCCCAAAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240606134629_3obw TTTTCTGCTTTTTGGGAGAATCATCTTCCATG(+AAAAAA)GATCCTCTCAGGCAGACAGAATAAAGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606134628_hs4e GAAGGCTTTCTACTCCTTAACAGGTACAGTTG(+AAAAAA)GATCCATCGCTGATGGTGTTTCTTTAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606134626_cq92 GGGATGGGGCTAAAGAAACACCATCAGCGATG(+AAAAAA)GATCCAACTGTACCTGTTAAGGAGTAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606134625_5h6b GGAGGGATGCAGACTGAGGCTGCTCAATTCTG(+AAAAAA)GTACCCCATAGTTCCTGTTGGCCTTAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606134623_lgmm GTTTTGGCTTTAAGGCCAACAGGAACTATGGG(+AAAAAA)GTACCAGAATTGAGCAGCCTCAGTCTGCATC NGG 10 8 17 8 24 0 100 5 10
    240606134622_465l AACAGCATATCCAACAAGGGAGCTAAGAACTG(+AAAAAA)GATCCATGAGCATCCTAAGGATGAGGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606134621_zwuk CATGACCAGGCCTCATCCTTAGGATGCTCATG(+AAAAAA)GATCCAGTTCTTAGCTCCCTTGTTGGATATG NGG 10 8 17 8 24 0 100 5 10
    240606134619_iawu GGGGATCTGTCACCTTCTTTTGGCTTCATTGG(+AAAAAA)GTACCTTAGCACATGTGGCATATATAGATAT NGG 10 8 17 8 24 0 100 5 10
    240606134618_5ce5 TCTTTATATCTATATATGCCACATGTGCTAAG(+AAAAAA)GTACCCAATGAAGCCAAAAGAAGGTGACAGA NGG 10 8 17 8 24 0 100 5 10