Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134617_sdr8 GAATGTTAGATATTTTTTCCATGGGGTCAAAG(+AAAAAA)GTACCTAAGTATATGATTGCGAGTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240606134616_24up CCCTATGTTTCCACTCGCAATCATATACTTAG(+AAAAAA)GTACCTTTGACCCCATGGAAAAAATATCTAA NGG 10 8 17 8 24 0 100 5 10
    240606134614_p7wy TTTCCTCTCCTGTCAGGCCTTTCTCAGGGTAG(+AAAAAA)GTACCTGCCCTGAGACTATCACATGGTCATT NGG 10 8 17 8 24 0 100 5 10
    240606134612_9p21 CCAGAAATGACCATGTGATAGTCTCAGGGCAG(+AAAAAA)GTACCTACCCTGAGAAAGGCCTGACAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606134611_hxjr CCTTTCTCAGTCACAAGCCATGACGACATGGG(+AAAAAA)GATCCGGACGCTTTGTGCTCTTAGCTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606134609_1qrp CGGCCACAGAAGCTAAGAGCACAAAGCGTCCG(+AAAAAA)GATCCCCATGTCGTCATGGCTTGTGACTGAG NGG 10 8 17 8 24 0 100 5 10
    240606134606_5rm3 GAAGCTCAGGCAGAAAGCCAACGGCTCCCAAG(+AAAAAA)GATCCACCCTGAGGAGCTAAGCAACGCGCTC NGG 10 8 17 8 24 0 100 5 10
    240606134604_gded TGAGCGAGCGCGTTGCTTAGCTCCTCAGGGTG(+AAAAAA)GATCCTTGGGAGCCGTTGGCTTTCTGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606134603_s9vx ATGCAACACTGGGTGTTCACCTGCCTTACTGG(+AAAAAA)GATCCCATGTGTTTAGTAATTTAACGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606134601_6uns TTCAGCTGGACGTTAAATTACTAAACACATGG(+AAAAAA)GATCCCAGTAAGGCAGGTGAACACCCAGTGT NGG 10 8 17 8 24 0 100 5 10