Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134528_cwoz ACCCTCAGCCTTGTCAACAGCTGAAAAATCTG(+AAAAAA)GTACCCAAAAGTCAAGAGGGTTTAAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240606134526_efxo TTTATTAAGTCTTAAACCCTCTTGACTTTTGG(+AAAAAA)GTACCAGATTTTTCAGCTGTTGACAAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606134525_m140 AAGAAAAGGGATGTCCAGTGCAGAGTCTACAG(+AAAAAA)GATCCAAAGTGAAGAACTGCAGTGGACACTC NGG 10 8 17 8 24 0 100 5 10
    240606134524_bdde TGAATGAGTGTCCACTGCAGTTCTTCACTTTG(+AAAAAA)GATCCTGTAGACTCTGCACTGGACATCCCTT NGG 10 8 17 8 24 0 100 5 10
    240606134522_qbkv ATGGTATGAATTGTATTGTAATGCTAAATCTG(+AAAAAA)GTACCTGAAGGTCTAGGCCTGTGAGTGAATT NGG 10 8 17 8 24 0 100 5 10
    240606134521_jryn GTGAGAATTCACTCACAGGCCTAGACCTTCAG(+AAAAAA)GTACCAGATTTAGCATTACAATACAATTCAT NGG 10 8 17 8 24 0 100 5 10
    240606134520_h9uk TTTGTAAGCCAGTTGGGGATAAGCTTATATTG(+AAAAAA)GTACCACATGGGAGAAAAAGAACCAGGACCT NGG 10 8 17 8 24 0 100 5 10
    240606134519_jxnp ATTCAAGGTCCTGGTTCTTTTTCTCCCATGTG(+AAAAAA)GTACCAATATAAGCTTATCCCCAACTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606134517_tz0x CTTGTGAAGCTCAGGAGCTGTGATCATATCAG(+AAAAAA)GTACCAGCATATGCCTTCTCAGTCATGATCC NGG 10 8 17 8 24 0 100 5 10
    240606134516_lf2v AGACAGGATCATGACTGAGAAGGCATATGCTG(+AAAAAA)GTACCTGATATGATCACAGCTCCTGAGCTTC NGG 10 8 17 8 24 0 100 5 10