Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134542_79gr CTTTGTCCTCGTCGAGCTGCTCTGAGCTGTGG(+AAAAAA)GATCCTGTGAACAACTGTCGTCTTTCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606134541_1hwr TGAAGAGAAGGAAAGACGACAGTTGTTCACAG(+AAAAAA)GATCCCACAGCTCAGAGCAGCTCGACGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606134539_27tn CCAAAATGCCCGTCCTATAGAGTGTCTGATTG(+AAAAAA)GATCCTCTGTTGCGTATTCTGATGGTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606134538_g5i6 ACCAGCAGGCACCATCAGAATACGCAACAGAG(+AAAAAA)GATCCAATCAGACACTCTATAGGACGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606134537_ykpw GCTGTTGTCAGTCTCCATGAAAGCTCTAGCTG(+AAAAAA)GTACCTTGGGTCTTGTCCTGACACCCGTATC NGG 10 8 17 8 24 0 100 5 10
    240606134534_v9s7 TCACAGATACGGGTGTCAGGACAAGACCCAAG(+AAAAAA)GTACCAGCTAGAGCTTTCATGGAGACTGACA NGG 10 8 17 8 24 0 100 5 10
    240606134533_kudf GTGTCTCATAAAGCAGCTGTTCAGGATGTGTG(+AAAAAA)GATCCTCTGCCATTGCTCTCCCGCGTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240606134532_w1ls GAGCTTCCCCACGCGGGAGAGCAATGGCAGAG(+AAAAAA)GATCCACACATCCTGAACAGCTGCTTTATGA NGG 10 8 17 8 24 0 100 5 10
    240606134530_5pz1 CCCTATGTTTCCACTCGCAATCATATACTTAG(+AAAAAA)GTACCTTTGACCCCATGGAAAAAAATATCTA NGG 10 8 17 8 24 0 100 5 10
    240606134529_awkd AATGTTAGATATTTTTTTCCATGGGGTCAAAG(+AAAAAA)GTACCTAAGTATATGATTGCGAGTGGAAACA NGG 10 8 17 8 24 0 100 5 10