Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134717_18j8 ACACTCAGAAGTTGCATTCAACAGGGCCACTG(+AAAAAA)GATCCCCTTGGGATTCGGTGCCAGATATTAC NGG 10 8 17 8 24 0 100 5 10
    240606134715_6uy2 ACCCTGTAATATCTGGCACCGAATCCCAAGGG(+AAAAAA)GATCCAGTGGCCCTGTTGAATGCAACTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606134713_v3co GGTGCACATCCTATGCCGCACAGCGGTGAGTG(+AAAAAA)GATCCCGGCCAAGAGAATACTCCAGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606134711_rhx1 GATGTCTGAGACTGGAGTATTCTCTTGGCCGG(+AAAAAA)GATCCACTCACCGCTGTGCGGCATAGGATGT NGG 10 8 17 8 24 0 100 5 10
    240606134709_wxk9 TTAGGCAAAGTTAATAGGGTATCTGGCATTGG(+AAAAAA)GATCCTACTGACTGCCATTCAGTAAGCCAAT NGG 10 8 17 8 24 0 100 5 10
    240606134708_ez89 TAACTATTGGCTTACTGAATGGCAGTCAGTAG(+AAAAAA)GATCCCAATGCCAGATACCCTATTAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240606134706_gc9g TTCAGGGAAAAAGAAAACATGTTTTACCTAGG(+AAAAAA)GATCCTTCTGAACAAAATGGCTGTCTGTACT NGG 10 8 17 8 24 0 100 5 10
    240606134704_jkl0 AAAAGAGTACAGACAGCCATTTTGTTCAGAAG(+AAAAAA)GATCCCTAGGTAAAACATGTTTTCTTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606134702_ry8i ATCAGGGGTTGGGTCCTGGACGACAACACAGG(+AAAAAA)GATCCTTTGAGACCCTCTTAAGTGGGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240606134701_twrf CATACTTTGGCCCACTTAAGAGGGTCTCAAAG(+AAAAAA)GATCCCTGTGTTGTCGTCCAGGACCCAACCC NGG 10 8 17 8 24 0 100 5 10