Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134802_1ls4 TGACCAAGTTTGCAGCATTGCTGGTGATGTGG(+AAAAAA)GATCCGTGGGGTTTTTGTTTCCCAAGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606134759_qawo GAAACCCTGTCTTGGGAAACAAAAACCCCACG(+AAAAAA)GATCCCACATCACCAGCAATGCTGCAAACTT NGG 10 8 17 8 24 0 100 5 10
    240606134758_0n3y GTGACTTAAATGGGGTCTCCCGGTTTCAAGTG(+AAAAAA)GATCCTCACTCCTGCCCGTTCTTAGCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606134757_dj4h GGAGGTCCCTGCTAAGAACGGGCAGGAGTGAG(+AAAAAA)GATCCACTTGAAACCGGGAGACCCCATTTAA NGG 10 8 17 8 24 0 100 5 10
    240606134755_b3zo GATGCTGCACTATTGAACAAGAAAACAGTAAG(+AAAAAA)GTACCACCAACACAACCCTCTTGTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606134754_4sy1 TTTTTTCCTTTGACAAGAGGGTTGTGTTGGTG(+AAAAAA)GTACCTTACTGTTTTCTTGTTCAATAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240606134753_r1ub GATGGACCTCACCCCTTTTCCACAGGTCACTG(+AAAAAA)GATCCCACTGTCACCCTCGGTGCTTACACAT NGG 10 8 17 8 24 0 100 5 10
    240606134751_tw6e CTGAAATGTGTAAGCACCGAGGGTGACAGTGG(+AAAAAA)GATCCAGTGACCTGTGGAAAAGGGGTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606134749_sp2p AAATCACTGCCCCATACTCAAACGACTCCCAG(+AAAAAA)GTACCCATTACTAGAAGTTTCTAAGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240606134747_vpyi GGAGCCTGTGACTTAGAAACTTCTAGTAATGG(+AAAAAA)GTACCTGGGAGTCGTTTGAGTATGGGGCAGT NGG 10 8 17 8 24 0 100 5 10