Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134833_jah4 TTTAAGCCCACCTGGTCACCCATCAAAGTCCG(+AAAAAA)GATCCTGTCTTTCCCCGTTTACCCTTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240606134832_x0uh GGAGGGTGGGAAGGGTAAACGGGGAAAGACAG(+AAAAAA)GATCCGGACTTTGATGGGTGACCAGGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606134830_pj2n GCTTTATTCATTATTGACATTTGTAGTTGAGG(+AAAAAA)GATCCGGGGGTTCAATTTATTTTGATTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606134827_8wqw AAAAAAAAAAATCAAAATAAATTGAACCCCCG(+AAAAAA)GATCCCTCAACTACAAATGTCAATAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240606134826_qilp TGGCCACCACCTCCCCCGGACTCTACCTCAAG(+AAAAAA)GATCCACCTCAAACCAAGCCTGACCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606134824_asdv GGTGATGCCTGGGTCAGGCTTGGTTTGAGGTG(+AAAAAA)GATCCTTGAGGTAGAGTCCGGGGGAGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240606134823_pk71 AAGGAGGCGACGCAGAGGCACTGGGGTACAGG(+AAAAAA)GTACCAAATGAAAGAGAGAGAGTCTTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606134821_rm8n CTCCATTCTCAAGACTCTCTCTCTTTCATTTG(+AAAAAA)GTACCCTGTACCCCAGTGCCTCTGCGTCGCC NGG 10 8 17 8 24 0 100 5 10
    240606134819_mhu5 AAAAATGGAATGAGCCACCGGCAGGTGCCCTG(+AAAAAA)GTACCAGAAGGACTGACTGCCGTGAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240606134818_amde GATAGTGTCTCTCACGGCAGTCAGTCCTTCTG(+AAAAAA)GTACCAGGGCACCTGCCGGTGGCTCATTCCA NGG 10 8 17 8 24 0 100 5 10