Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134936_fyxp TCTGCCTCGAACTAATTCATCTCACGCTGTTG(+AAAAAA)GTACCGAGTGTGAGGCTCAGCGACTGGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606134935_97uw TGATTTTCTCCAGTCGCTGAGCCTCACACTCG(+AAAAAA)GTACCAACAGCGTGAGATGAATTAGTTCGAG NGG 10 8 17 8 24 0 100 5 10
    240606134934_f4eu TCCCTTGCCTAAGGCAGAGCCGTTTCCTTCAG(+AAAAAA)GTACCACACAAGCAATTTGCAGACCTGACTC NGG 10 8 17 8 24 0 100 5 10
    240606134932_3nj2 TTTCTGAGTCAGGTCTGCAAATTGCTTGTGTG(+AAAAAA)GTACCTGAAGGAAACGGCTCTGCCTTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606134930_m1lz ATAGAAAAGGGTGGTATCTGGGAGTCTAAGGG(+AAAAAA)GTACCTTTTCATCTGACCTACTGACCAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606134929_hks6 CTATGACTTTGGTCAGTAGGTCAGATGAAAAG(+AAAAAA)GTACCCCTTAGACTCCCAGATACCACCCTTT NGG 10 8 17 8 24 0 100 5 10
    240606134927_tvqc TCCATGCTACCTACCAATAAAACCAGGCCCTG(+AAAAAA)GTACCAAAGGCCTGTGGAGTACTGGGCACTG NGG 10 8 17 8 24 0 100 5 10
    240606134925_f9l3 CAGCGCAGTGCCCAGTACTCCACAGGCCTTTG(+AAAAAA)GTACCAGGGCCTGGTTTTATTGGTAGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240606134923_wumq GAGTGCTGATAAAGGCATCTGGGGGCCCAGAG(+AAAAAA)GATCCCAGTAGAGACTGCTTGCTCTATGTAT NGG 10 8 17 8 24 0 100 5 10
    240606134921_mgv2 CTGTAATACATAGAGCAAGCAGTCTCTACTGG(+AAAAAA)GATCCTCTGGGCCCCCAGATGCCTTTATCAG NGG 10 8 17 8 24 0 100 5 10