Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140240_z149 TTGTTGACTCGGGCAGAAACTCCATAGCACAG(+AAAAAA)GTACCTGACGAGTGTGGAGAAGACCTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606140239_757m CATGGATTTGGGGATATTTTCCAGCTAGAATG(+AAAAAA)GATCCAGAATTGAATGGTGCTTAAATTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606140237_9w9m TTATTTCTCAATTTAAGCACCATTCAATTCTG(+AAAAAA)GATCCATTCTAGCTGGAAAATATCCCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240606140236_ukgb GGTATTTTCAGCTGCAGTTCTCCTCCTCTCTG(+AAAAAA)GATCCTGGCTTGGGGTGCTTTTCCAGCATCC NGG 10 8 17 8 24 0 100 5 10
    240606140234_3056 ACTGTGGATGCTGGAAAAGCACCCCAAGCCAG(+AAAAAA)GATCCAGAGAGGAGGAGAACTGCAGCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240606140232_k4fc CTATTAGGAGAGTAGATGTTAATGTGTTTTGG(+AAAAAA)GTACCCATCTTTGCAACATGTCTGATCCACG NGG 10 8 17 8 24 0 100 5 10
    240606140231_tlmq TGGCACGTGGATCAGACATGTTGCAAAGATGG(+AAAAAA)GTACCCAAAACACATTAACATCTACTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606140229_pxia CTATTAGGAGAGTAGATGTTAATGTGTTTTGG(+AAAAAA)GTACCCATCTTTGCAACATGTCTGATCCACG NGG 10 8 17 8 24 0 100 5 10
    240606140228_o08x TGGCACGTGGATCAGACATGTTGCAAAGATGG(+AAAAAA)GTACCCAAAACACATTAACATCTACTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606140226_dble CTATTAGGAGAATAGATGTTAATGTGTTTTGG(+AAAAAA)GTACCCATCTTTGCAACATGTCTGATCCAAG NGG 10 8 17 8 24 0 100 5 10